欧洲标准局 参考物质 应用说明 ERM-AD623 application note.pdfVIP

Application Note ® ERM -AD623 Author: Liesbet Deprez European Commission – Joint Research Centre Institute for Reference Materials and Measurements (IRMM) Retieseweg 111, 2440 Geel, Belgium Email: jrc-irmm-info@ec.europa.eu Use of ERM-AD623 for the Quantification of BCR-ABL1 transcripts The certified reference material (CRM) ERM-AD623 [1] is intended to be used for the calibration of quantitative real time polymerase chain reaction (qPCR) assays measuring the BCR-ABL1 b3a2 transcript levels in cDNA samples from leukaemia patients. INTRODUCTION INTENDED USE OF ERM-AD623 The fusion gene BCR-ABL1 results from a genetic ERM-AD623 is intended for the calibration of translocation joining two genes (c-abl oncogene 1 qPCR assays quantifying the BCR-ABL1 b3a2 [ABL1] and breakpoint cluster gene [BCR]) and is transcript in relation to the transcript of 1 of 3 the primary cause of chronic myeloid leukaemia control genes (CG): ABL1, BCR , or glucuronidase (CML). Breakpoints can occur at different beta (GUSB). Several qPCR assays with different positions within the genes and the two major probe/primer sets or PCR instruments can be transcripts are called b2a2 and b3a2 (joining exon