微藻常用培养基之f／2.docVIP

微藻常用培养基之f／2 微藻常用培养基之1/2 f f/2 Medium Stocks per litre (1) Trace elements (chelated) Na2 EDTA 4.16 g FeCl3.6H2O 3.15 g CuSO4.5H2O 0.01 g ZnSO4.7H2O 0.022 g CoCl2.6H2O 0.01 g MnCl2.4H2O 0.18 g Na2MoO4.2H2O 0.006 g (2) Vitamin mix Cyanocobalamin (Vitamin B12) 0.0005 g Thiamine HCl (Vitamin B1) 0.1 g Biotin 0.0005 g Medium per litre NaNO3 0.075 g NaH2PO4.2H2O 0.00565 g Trace elements stock solution (1) 1. 0 ml Vitamin mix stock solution (2) 1. 0 ml Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or HCl. Sterilise by autoclaving for 15 minutes at 15 p