叶酸靶向紫杉醇纳米胶束抑制小鼠H22肝癌肺转移的研究.docx

叶酸靶向紫杉醇纳米胶束抑制小鼠H22肝癌肺转移的研究 摘要 目的：探讨叶酸靶向纳米胶束在抑制小鼠H22肝癌肺转移中的作用及机制。 方法：制备大小均一、稳定性好的叶酸靶向纳米胶束，并测试其在肝癌细胞H22中的毒性。采用小鼠模型，分别给予叶酸靶向纳米胶束、普通纳米胶束和生理盐水控制组，观察其对小鼠肝癌肺转移的抑制效果以及对小鼠体内生化指标、免疫功能的影响，同时检测叶酸靶向纳米胶束的肿瘤组织分布情况。 结果：叶酸靶向纳米胶束对H22细胞有明显的毒性作用，IC50为6.5 μg/mL，普通纳米胶束的毒性较小，IC50为14.5 μg/mL。在小鼠模型中，与生理盐水控制组相比，叶酸靶向纳米胶束组和普通纳米胶束组的肝癌肺转移数量均明显减少，其中叶酸靶向纳米胶束组抑制效果更明显。叶酸靶向纳米胶束组和普通纳米胶束组对小鼠体内生化指标、免疫功能的影响在可接受范围内，而叶酸靶向纳米胶束能够更多地积聚在肿瘤组织内，增强对肿瘤的抑制作用。 结论：叶酸靶向纳米胶束能够抑制小鼠H22肝癌肺转移，其作用机制可能与通过靶向细胞表面的叶酸受体，使其更好地积聚在肿瘤组织内，增强抑制作用有关。同时，叶酸靶向纳米胶束具有较好的生物相容性和安全性。因此，叶酸靶向纳米胶束可望成为一种新型的肝癌肺转移治疗策略。 关键词：叶酸靶向；纳米胶束；肝癌肺转移 Abstract Objective: To investigate the effect and mechanism of targeted folate nanoparticles in inhibiting the lung metastasis of H22 hepatocellular carcinoma in mice. Methods: Folate-targeted nanoparticles with uniform size and good stability were prepared, and their toxicity in H22 hepatocellular carcinoma cells was tested. A mouse model was used, and folate-targeted nanoparticles, normal nanoparticles, and physiological saline control were given, and the inhibitory effect on the lung metastasis of H22 hepatocellular carcinoma in mice was observed, as well as the effect on the biochemical indicators and immune function in mice. The distribution of folate-targeted nanoparticles in tumor tissues was also detected. Results: Folate-targeted nanoparticles had obvious toxicity to H22 cells, with IC50 of 6.5 μg/mL, and normal nanoparticles had less toxicity, with IC50 of 14.5 μg/mL. In the mouse model, compared with the physiological saline control group, both the folate-targeted nanoparticle group and the normal nanoparticle group had significantly reduced numbers of lung metastases from hepatocellular carcinoma, with the folate-targeted nanoparticle group having a more significant inhibitory effect. There was acceptable impact on the biochemical indicators and immune function in both the folate-targeted nanoparticle group and the normal nanoparticle group, while folate-targeted nanoparticles were able to accumulate mo