(1.4.7)--广西南宁市柑橘木虱虫生真菌种类调查.pdfVIP

环境昆虫学报 Journal of Environmental Entomology ISSN 1674-0858,CN 44-1640/Q 《环境昆虫学报》网络首发论文 广西南宁市柑橘木虱虫生真菌种类调查 摘要：自柑橘木虱Diaphorina citri 体上分离虫生真菌，通过形态特征和ITS 序列分析，鉴定虫生真菌的种 类；通过致病性测定，明确昆虫病原真菌种类。结果发现，自死体柑橘木虱上分离到18 个虫生真菌菌株， 隶属4 种真菌；自活体上分离到985 个虫生真菌菌株，隶属25 种真菌。致病性测定结果发现，仅4 种真菌 对柑橘木虱成虫有致病性，包括刀孢蜡蚧菌Lecanicillium psalliotae 、球孢白僵菌Beauveria bassiana 、爪哇 虫草Cordyceps javanica 和淡紫紫孢菌Purpureocillium lilacinum ，该4 种真菌的分生孢子悬浮液（浓度5×107 个/mL ）接种柑橘木虱成虫后10 d 的累计死亡率分别为100%、100%、98.89%和43.33% 。其中爪哇虫草仅 自死体柑橘木虱上分离到，刀孢蜡蚧菌仅在活体上分离到，球孢白僵菌和淡紫紫孢菌在死体和活体上均被 分离到。可见，柑橘木虱活体上的虫生真菌种类丰富。生产上应加强保护和利用昆虫病原真菌，提高其对 柑橘木虱的自然抑制力。 关键词：柑橘木虱；虫生真菌；昆虫病原真菌；种类 中图分类号：Q968.1;S433 文献标识码：A Investigation on species of entomogenous fungi from Diaphorina citri in Nanning, Guangxi HUANG Yuan-Teng-Ji, ZHU Wen-Qian, WANG Zheng-Xian, HUANG Yi-Man, LIAO Yong-Mei (College of Agriculture, Guangxi University, Nanning 530004, China) Abstract: Entomogenous fungi were isolated from Diaphorina citri and their species were identified by morphological characteristics and ITS sequence analysis; the species of entomopathogenic fungi were determined by pathogenicity assay. The results showed that 18 entomogenous fungal strains were isolated from corpse of Diaphorina citri , belonging to 4 species of fungi; 985 entomogenous fungal strains were isolated from living Diaphorina citri , belonging to 25 species of fungi. The results of pathogenicity assay showed that only 4 species of fungi were entomopathogenic fungi of Diaphorina citri , including Lecanicillium psalliotae , Beauveria bassiana, Cordyceps javanica and Purpureocillium lilacinum . The cumulative mortality of Diaphorina citri adult were 100%, 100%, 98.89% and 43.33%, 10 days after inoculated with 基金项目：广西科技重大专项（桂科A） 作者简介：黄元腾吉，男，硕士，研究方向为植物病理学，E-mail ：54