中国农业大学 生物化学第19章 基因工程简介.pptVIP

In vitro development of chicken-rabbit interclass cloned embryos Karyotype Analysis of Reconstructed Blastocyst Nuclear DNA Analysis avian feather keratin gene D P1 5‘-GGAGAAGGTCCAGGGCTGACTTTA-3‘ P2 5‘-ACTTCTCTTGGCAAACATGCAACC--3‘ P3 5‘---GCGTCCACCTCATCCTTAGCAG---3 Electrophoretic analysis of PCR product using specific avian feather keratin Lane 1, markers(100bp ladder); Lane 2, chichen donor cells; Lane 3, 8-cell cloned embryo; Lane 4, blastocyst stage; Lane 5, morula stage; Lane 6, water (negative control) ; Lane 7, rabbit somatic cells; TF, target fragment. Mitoc