eftem光谱成像教程.pdfVIP

1. Introduction The EFTEM Spectrum Imaging package allows you to collect spectrum images one ne at a time from a microscope equipped with an imaging filter. This quick reference summarizes the capabilities of version 1.0 of the software. 2. Acquisition When you have tuned your imaging fil nd have a sample in ce you can acquire an EFTEM spectrum image. To colle pectrum image 1. Choose Setup on the EFTEMSI . Enter the CCD binning, integration time and energy range to sample. The energy range can be specified either by entering starting and finishing energies or by entering a starting energy, the number of nes, and a step size. The d an be acquired from high energy-loss to low energy-loss if desired by specifying a negative step size and a finishing energy numerically less than the starting energy. 2. Choose Acquire on the EFTEMSI . Two images appear. The leftmost is the spectrum image, the rightmost is the current ne being acquired. Acquisition will continue until all nes have been collected at which point the “current ne” will disappear and a beep will sound. 3. Artifact Correction The EFTEM spectrum image will most likely suffer from x-ray hits and a drift of the sample during the acquisition. The x-ray hits will appear as occasional extreme values in the image nes of the spectrum image. If the sample drifted during the experiment then the image nes will not line up with each other and spectra drawn from the spectrum image will contain artifacts. The software can help you to reduce or eliminate these problems. 3.1. Removing x-rays To remove x-rays hits from a spectrum image 1. Choose Remove x-rays from the Artifact correction sub . The software will check each image ne of the spectrum image for es that lie more than 10 standard deviations above the the local median. The number of standard deviat