高危HPV病毒阳性宫颈上皮细胞P16、Ki-67的表达 .pdfVIP

高危 HPV 病毒阳性宫颈上皮细胞 P16、Ki-67 的表达 帅丽华;王敏;胡志坚;李婷;黎美仁;陈萍 【摘要】 目的：研究人乳头瘤病毒(HPV)高危型 HPV-DNA 阳性宫颈上皮细胞不 典型增生和低级别宫颈上皮内瘤变(Low-grade Squamous Intraepithelial Lesion ，LSIL)患者 pl6、Ki-67 表达情况。方法用 Hybrid CaptureⅡ（HC2 ）或 FQ-PCR 体外扩增技术检测宫颈上皮细胞高危型 HPV ﹣DNA ，同时行液基细胞学 检查。对 97 例高危型 HPV-DNA 阳性且液基细胞学诊断为不典型增生(ASCUS)或 CINⅠ或 CINⅡ的患者，行阴道镜检查取可疑宫颈组织标本多点活检并 EnVision 两步法进行免疫组化标记 pl6、Ki-67。结果 97 例宫颈上皮细胞高危 HPV-DNA 阳性患者中，宫颈组织 CINⅡ级 38 例，CINⅠ级 35 例，炎症和（或） ASCUS 24 例。P16 标记结果：3 例 +++ ，30 例 ++ ，39 例 + ，P16 阳性表达率为 74.2% ； Ki-67 标记结果：3 例 +++ ，30 例 ++ ，30 例 + ， Ki-67 阳性表达率为 64.9%。 炎症和（或）ASCUS、CINⅠ、CINⅡ三者比较，P16 和 Ki-67 的阳性率和阳性程 度具有极显著性差异（ P0.01 ）。结论高危HPV 病毒阳性宫颈上皮细胞随着病情 严重程度增加 pl6、Ki-67 的阳性率和阳性程度均升高，pl6、Ki-67 的检测对 CIN 的准确分级有利。%Objective To study the expression of P16 and Ki-67 of patients with Human papillomavirus (HPV) type of high-risk HPV-DNA and atypical hyperp-lasia or Low-grade Squamous Intraepithelial Lesion (LSIL). Methods Test high-risk HPV-DNA positive rate of cervical epithelial cells with Hybrid Capture Ⅱ (HC2) or Fluorescent quantitati ve polymerase chain reaction (FQ-PCR),and LPT fluid based cytology at the same time. For 97 abnormal patients whose high-risk HPV-DNA tested positive and liquid based cytology diagnosis of ASCUS or CINⅠor CINⅡ,with Colposcopy obtain suspicious tissue of cervical multi-spot biopsy to diagnose and EnVision two-step immunohistochemical marks pl6,Ki-67. Results In 97 patients whose high-risk HPV-DNA tested positive,cervical tissue CINⅡ38 cases,CINⅠ 35 cases, inflammation or ASCUS 24 cases. Marked outcomes of P16:+++3 cases,++30 cases,+39 cases. The positive rate of P16 was 74.2%. Marked outcomes of Ki-67:+++3 cases,++30 cases,+30 cases,The positive expression of Ki-67 was 64.9%. There were extremely significant difference in the positive rate and posi-tive degree of P16 and Ki-67 by comparing inflammation and(or) ASCUS,CINⅠ,CINⅡ. Conclusion The Positive rate and posi-tive degree of pl6 and Ki-67 of abnormal cervical epithelial