美国瓦里安 工作站教程现场培训资料basic_dh.ppt

Basic Principles of Data HandlingStar Workstation Ver 5 LearningData Handling Data Handling is a complex program with many settings that control how chromatographic signals are analyzed Data Handling Functional OverviewData Handling can be easily understood by breaking it down into its fundamental operations Data Handling Functional Overview Peak DetectionPeak Detection is the operation of finding a chromatographic signal from an eluting compound within the noise coming from the detector.Peak Detection works off a change in the slope or derivative of the detector signal Factors AffectingPeak DetectionBaseline Noisesampled automatically before injection, orfixed value can be set in methodDetector or ADC board data sampling rate (detector or ADC method section)Signal to Noise Ratio (DH time event)Peak Width Setting (DH time event)Inhibit Integrate (DH time event) Noise Noise is measured during the “monitor” stage of the ADC board and is used to control the sensitivity of the integrator to a peak start or end Chromatographic Noise is generally expressed as “Peak to Peak” noise NOISE uV/sec Signal / NoisePeak sensitivity is adjusted through s/n time eventsNoise may be established by:Monitoring before every runBefore the first of a seriesUser defined value The analog output from the detector is sampled at a maximum of 40 Hz (40 data points/second). This rate can be bunched and averaged based on the “bunch rate” in the Detector Information window and “width” in the Time Events window of the method. Detector Bunch Rate  Effect of Bunch RateSampling Rate = 40/8 = 5HzSampling Rate = 40/4 = 10 HzExceeds minimum forpeak start/end ie. S/Nin uVBunch Rate = 4Bunch Rate = 8 Peak Width Time Event (WI)Used to time program the sampling rate used for peak detectionWI = 2WI = 4WI = 8 Peak Width (WI)WI =2WI =8 Integration InhibitTurns off the integration during the specified time and/or terminates a peak Two Time Events which affect the detection