中药千层塔中生物碱成分的色谱分离方法研究的中期报告.docxVIP

中药千层塔中生物碱成分的色谱分离方法研究的中期报告 摘要： 本研究旨在通过色谱分离技术研究中药千层塔中生物碱成分的分离和鉴定方法。首先采用薄层色谱对千层塔进行初步分离，并利用紫外光谱和显色剂观察分离结果。然后，在高效液相色谱条件下，选择C18反相柱和水/乙腈组成的移动相进行分离，并采用荧光检测器检测各组分的浓度和保留时间。初步结果表明，利用高效液相色谱分离条件可以有效地分离和鉴定千层塔中的生物碱成分。 关键词：中药；千层塔；生物碱；色谱分离；高效液相色谱 Abstract: The objective of this study is to investigate the separation and identification methods of alkaloid components in Chinese medicine Qiancengta using chromatographic separation techniques. First, thin-layer chromatography was used for preliminary separation of Qiancengta, and the separation results were observed using UV spectrophotometry and color reagents. Then, under the condition of high-performance liquid chromatography, C18 reverse-phase column and water/acetonitrile composition of mobile phase were selected for separation, and fluorescence detector was used to detect the concentration and retention time of each component. Preliminary results show that the alkaloid components in Qiancengta can be effectively separated and identified using high-performance liquid chromatography separation conditions. Keywords: Traditional Chinese medicine; Qiancengta; Alkaloids; Chromatographic separation; High-performance liquid chromatography.