淇澳岛红树林细菌群落结构分析及芘降解菌生长条件研究的中期报告.docxVIP

淇澳岛红树林细菌群落结构分析及芘降解菌生长条件研究的中期报告 摘要： 本研究旨在分析淇澳岛红树林细菌群落的结构和研究能够对芘有较好降解能力的菌株的生长条件。通过实验室分离和鉴定得到多种细菌菌株，经过对全长16S rDNA序列进行分析，发现细菌群落主要由革兰氏阴性菌组成，其中以β-蛋白杆菌属和绿脓杆菌属为主。 在芘降解菌的筛选中，发现一株能够高效降解芘的蓝绿链球菌，经过对影响该菌生长的因素的研究发现，该菌的最适生长条件为pH 7.0，温度为30℃，添加0.5%葡萄糖和0.1%酵母提取物时生长最佳。进一步对该菌进行发酵实验，结果表明在最适生长条件下，该菌对芘的降解效率高达83.5%。 关键词：淇澳岛；红树林；细菌群落；芘降解菌；生长条件；蓝绿链球菌 Abstract: The objective of this study was to analyze the structure of bacterial communities in the mangrove forests of Qiao Island, and to investigate the growth conditions of bacterial strains with strong ability to degrade pyrene. A variety of bacterial strains were isolated and identified in the laboratory, and analysis of the full-length 16S rDNA sequence revealed that the bacterial communities were primarily composed of Gram-negative bacteria, with Burkholderia and Pseudomonas as the predominant genera. In the screening of pyrene degradation bacteria, a highly efficient strain of cyanobacterial cocci capable of degrading pyrene was found. Through research on factors affecting the growth of the strain, it was found that the optimal growth conditions were pH 7.0, temperature 30℃, and addition of 0.5% glucose and 0.1% yeast extract. Further fermentation experiments on the strain showed that under the optimal growth conditions, the degradation efficiency of pyrene reached up to 83.5%. Keywords: Qiao Island; Mangrove forest; Bacterial communities; Pyrene degradation bacteria; Growth conditions; Cyanobacterial cocci