青蒿琥酯影响Hela细胞免疫抑制物质分泌的研究的中期报告.docxVIP

青蒿琥酯影响Hela细胞免疫抑制物质分泌的研究的中期报告 摘要 本研究旨在探讨青蒿琥酯对Hela细胞免疫抑制物质分泌的影响，并初步分析其可能的作用机制。采用细胞培养技术，将Hela细胞分为青蒿琥酯处理组和对照组，分别进行细胞培养并检测细胞培养液中的免疫抑制物质分泌水平。结果显示，相对于对照组，青蒿琥酯处理组细胞培养液中的免疫抑制物质分泌水平显著降低。同时，我们进行了Western Blotting技术检测，发现青蒿琥酯处理组中TGF-β和IL-10的表达明显下调，NF-κB和STAT3的表达明显上调。这些结果提示，青蒿琥酯可能通过抑制NF-κB和STAT3的激活，降低TGF-β和IL-10的表达水平，从而抑制Hela细胞免疫抑制物质的分泌。本次研究结果为深入探究青蒿琥酯的作用机制提供了重要线索。 关键词：青蒿琥酯；Hela细胞；免疫抑制物质；NF-κB；STAT3 Abstract The aim of this study is to explore the effects of artemether on the secretion of immune suppressive substances in Hela cells and preliminary analysis of its possible mechanism of action. The Hela cells were divided into two groups: artemether-treated group and control group, respectively, and then cell culture was performed and the levels of immune suppressive substances in the cell culture medium were measured. The results showed that the levels of immune suppressive substances in the cell culture medium of artemether-treated group were significantly lower than those in the control group. Western blotting was carried out and it was found that the expression of TGF-β and IL-10 in artemether-treated group was significantly down-regulated, while the expression of NF-κB and STAT3 was significantly up-regulated. These results suggest that artemether may inhibit the activation of NF-κB and STAT3, reduce the expression levels of TGF-β and IL-10, and thus inhibit the secretion of immune suppressive substances in Hela cells. The results of this study provide important clues for further elucidation of the mechanism of action of artemether. Keywords: artemether; Hela cells; immune suppressive substances; NF-κB; STAT3