Lab-1培养基的配制与灭菌.pptxVIP

Lab-1培养基的配制与灭菌

2Sterilization–destructionofallformsofmicrobiallife,includingendospores.PhysicalMethods—MoistheatAutoclavingAction-proteindenaturation. Veryeffectivemethodofsterilization;atabout15psi（Ib/in2）ofpressure(121oC[250oF]),allvegetativecellsandtheirendosporesarekilledinabout15min.

—DryheatDirectflamingexample:InoculationloopHot-airsterilizationBakinginoven Veryeffectivemethodofsterilization,butrequirestemperatureof170oC[340oF]forabout2hr.

—Filtration– Separationofbacteriafromsuspendingliquid.– Removesmicrobesbypassageofaliquidorgasthroughascreenlikematerial.Mostfiltersconsistofdiatomaceousearth,celluloseacetateornitrocellulose.– 0.45μmfiltersremovesmostbacteria,0.20μmmoreinclusive,virusesneed0.01μm.– Usefulforsterilizingliquids(enzymes,vaccines)thataredestroyedbyheat.

三 实验材料与器材培养基：牛肉膏、蛋白胨、琼脂、可溶性淀粉、葡萄糖、孟加拉红、链霉素、1mol/LNaOH、1mol/LHCl、KNO3、K2HPO4·3H2OMgSO4·7H2OFeSO4·7H2O.器材：玻璃器皿、天平、pH试纸、灭菌锅等

四操作步骤称量→溶解→调pH*→（过滤*）→分装*→加塞*→包扎→高压蒸汽灭菌高压蒸汽灭菌灭菌操作步骤首先将内层灭菌桶取出，再向外层锅内加入适量的水，使水面与三角搁架相平为宜。