高效液相色谱法测定奶粉中羟脯氨酸含量.pdfVIP

高效液相色谱法测定奶粉中羟脯氨酸含量

于淑新;赵连海;王丰琳;孙元社;唐涛;李彤

【摘要】Inthispaper,amethodformeasurementofhydroxyprolinein

milkpowderbyhighperformanceliquidchromatography(HPLC)withpre-

columnderivationwasestablished.2,4-dinitrofluorobenzene(DNFB)was

usedasderivativereagent,andtheseparationwasperformedonEliteAAK

C18column(250mm×4.6mmI.D.,5μm)withgradientelutionof0.

05mol/Lsodiumacetateandacetonitrile/water(50:50,V:V)at27℃.The

flowratewas1.2mL/minandthedetectionwavelengthwas360nm.

Methodvalidationwasperformed.Theresul