ASTM E1563-98(2012)美国材料与试验协会标准.pdfVIP

Designation:E1563−98(Reapproved2012)

StandardGuidefor

ConductingStaticAcuteToxicityTestswithEchinoid

Embryos1,2

ThisstandardisissuedunderthefixeddesignationE1563;thenumberimmediatelyfollowingthedesignationindicatestheyearof

originaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.A

superscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.

1.Scopeeffluents(seealsoGuideE1192),leachates,oils,particulate

1.1Thisguidecoversproceduresforobtaininglaboratorymatter,surfacewatersandsediments(AnnexA1).Renewal

dataconcerningtheacuteeffectsofatestmaterialonembryostestsmightbepreferabletostatictestsformaterialsthathave

andtheresultinglarvaeofechinoidembryos(seaurchinsandahighoxygendemand,arehighlyvolatile,arerapidlytrans-

sanddollars)duringstatic48-to96-hexposures.Theseformedbiologicallyorchemicallyinaqueoussolution,orare

procedureshavegenerallybeenusedwithU.S.EastCoastremovedfromtestsolutionsinsubstantialquantitiesbythetest

(ArbaciapunctulataandStrongylocentrotusdroebachiensis)chambersororganismsduringthetest.

(1)3andWestCoastspecies(Strongylocentrotuspurpuratus,S.1.4Resultsofacutetoxicitytestswithechinoidembryos

droebachiensis,andDendrasterexcentricus)(2).Thebasicshouldusuallybereportedasthe50%effectconcentration

proceduresdescribed