磷丙泊酚2二异丙基苯酚一种广泛使用静脉用于诱导维持全身空空.pdfVIP

Propofol-inducedInhibitionofCatecholamineRelease

IsReversedbyMaintainingCalciumInflux

LiHan,M.D.,M.Sc.,StephenFuqua,M.S.,QuanlinLi,M.D.,Ph.D.,LiyuZhu,M.D.,

XiaoyanHao,M.D.,M.Sc.,AiLi,M.D.,M.Sc.,SangeetaGupta,B.S.,RavinderSandhu,B.S.,

GyörgyLonart,Ph.D.,ShuzoSugita,Ph.D.

Background:propofol(2,6-diisopropylphenol)isoneofthemostfrequentlyusedanestheticagents.oneofthemainside

effectsofpropofolistoreducebloodpressure,whichisthoughttooccurbyinhibitingthereleaseofcatecholaminesfrom

sympatheticneurons.Here,theauthorshypothesizedthatpropofol-inducedhypotensionisnotsimplytheresultofsuppres-

sionofthereleasemechanismsforcatecholamines.

Methods:Theauthorssimultaneouslycomparedtheeffectsofpropofolonthereleaseofnorepinephrriggeredbyhigh

+2+

K-induceddepolarization,aswellasionomycin,byusingneuroendocrinepC12cellsandsynaptosomes.Ionomycin,aCa

ionophore,directlyinducesCa2+influx,thusbypassingtheeffectofionchannelmodulationbypropofol.

+

Results:propofoldecreaseddepolarization(highK)-triggerednorepinephrinerelease,whereasitreasedionomycin-triggered

releasefrombothpC12cellsandsynaptosomes.Thepropofol(30μM)-inducedreaseinnorepinephrinereleasetriggeredby

ionomycinwasdependentonboththepresenceandtheconcentrationofextracellularCa2+(0.3to10mM;n=6).Theenhance-

mentofnore