章先天免疫细胞适应性section 9 of innate immuinty20149节icells2014.pptx

Chapter10

Cellsofinnateimmunity;;CellsofInnateImmunity;

一.Monocyte-macrophage;Macrophagesarelargerthanmonocytes

andcontainmoreorganelles,especiallylysosomes.

;(一)Receptorsofmacrophages;PAMP;;1.PRR;PAMP:LPSandpeptidoglycan;Toll-likereceptor(TLR):11members.

Oncellmembrane:TLR1,2,4,5,6

TLR2:peptidoglycan,teichoicacid,lipopeptide.

TLR4:LPS,HSP60,teichoicacid.

Onorganellemembrane:TLR3,7,8,9

ds/ssDNA,CpGDNA;SolublePRRreceptors;MBLpathway;2.IgGFcreceptor（FcγR）：opsonizephagocytosisofmacrophage.

3.Complementreceptors：C3bR,C4bR.C3b,C4bbindingpathogensontheaminoendandreceptorsoncarboxylend,opsonizephagocytosis.

4.Cytokinereceptors:

Activation(IFN-γ,M-CSF,GM-CSF)andchemotrafficking(MIP-1α/β).

;(二)Biologicalfunctionsofmacrophages;1.KillingandClearanceofPathogens;Receptormediatedphagocytosis.

Oxygen-dependentkilling:

Reactiveoxygenintermediates(ROI):O2-,H2O2,1O2.,OH-

Reactivenitrogenintermediates(RNI):NO

Non-oxygen-dependentkilling:

Acidification

Enzymesandpeptide:lysozyme,defensin

;Digestionandclearance:

Proteinase,nuclease,phosphatase,esterase.

Exocytosis.

;Bactericidalagentsproducedorreleasedbyphagocytesontheingestionofmicroorganisms.Someofthemaretoxic;others,suchaslactoferrin,workbybindingessentialnutrientsandpreventingtheiruptakebythebacteria.Astheseagentsarealsotoxictohostcells,phagocyteactivationcancauseextensivetissuedamageduringaninfection.;2.Agprocessingandpresentation;3.Participatingininflammation;Whatisinflammation;4.Killingtargetcells;5.Immuneregulation;CellsofInnateImmunity;二.Neutrophils;三.Dendtriticcell;DendriticCell(DC);StatusofDC

;;1.Agprocessingandpresentation:

MostpowerfulAPC,whichactivatena?veTcells.

2.APCdeterminethetypeofimmuneresponses.

IL-4,IL-5---Th2differentiation,humoralimmunity.

IL-2,IL-12----Th1differe