高效液相色谱法课件.pptxVIP

;高效液相色谱法HPLC

HighPerformanceLiquidChromatography;高效液相色谱法HPLC

HighPerformanceLiquidChromatography;高效液相色谱法HPLC

HighPerformanceLiquidChromatography;高效液相色谱法HPLC

HighPerformanceLiquidChromatography;高效液相色谱法HPLC

HighPerformanceLiquidChromatography;液固色谱

Liquid-SolidChromatography;液固吸附色谱

Liquid-SolidChromatography;;化学键合相色谱

BondedPhaseChromatography;;化学键合相色谱

BondedPhaseChromatography;化学键合相色谱

BondedPhaseChromatography;反相离子对色谱RP-Ion-PairHPLC;化学键合相色谱

BondedPhaseChromatography;化学键合相色谱

BondedPhaseChromatography;化学键合相色谱

BondedPhaseChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;空间排斥（阻）色谱

SizeExclusionChromatography;Conditions:Column,VydacC18,5-mm,30nm(1mmID×100mm)

Flowrate,40mL/min

Mobilephase,A:0.05%FormicAcidinWater

B:0.05%FormicAcidinAcetonitrile

Lineargradientprogrammedfrom20%Bto100%B

over25min,holdat100%Bfor5min

Sample:mixtureof9proteinstandards;5-mLloop

Detection:SCIEXTAGA6000Etriplequadrupolemassspectrometry

equippedwithionsprayatmosphericpressureionsourcein

positiveionmode

1,substanceP(1-9),MW=1104,900pmoles;2,neurotensin,1673,150;

3,angiotensin,1296,385;4,somatostatin,1638,600;5,bovineinsulin,

5732,130;6,bovineglucagon,3482,720;7,mellitin,2846,175;8,myo-

Globin,16950,20;9,lactoglobulin-B,18277,245.;m-HPLC-MassSpectrometry

ofPeptidesandProteins;MassSpectrum

ofSubstancePfromPeak1;MassSpectrum

ofBovineInsulinfromPeak5;MassSpectrum

ofBovineGlucagon(胰增血糖素）fromPeak6;MixedMassSpectrumfromPeak8;Separationanddetectionofthepigments

innarcissus’leavesbym-HPLC-APCI-MS;Separationanddet