木犀草素对兔耳增生性瘢痕抑制作用机制的初步研究.docx

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木犀草素对兔耳增生性瘢痕抑制作用机制的初步研究

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李泰平肖红

[摘要]目的：初步研究木犀草素对兔耳增生性瘢痕的抑制作用及可能机制。方法：9只新西兰兔随机分为空白组、生理盐水对照组和药物干预组，每组3只，生理盐水对照组和药物干预组建立兔耳增生性瘢痕模型，每只兔左右耳各3個直径约0.7cm瘢痕创面，相距约1cm。生理盐水对照组给予生理盐水，药物干预组给予木犀草素乳膏，瘢痕组织于给药前和给药后40d取材，Masson染色后显微镜下观察各组瘢痕组织的病理变化，ELISA法检测瘢痕组织中转化生长因子-β（TGF-β）、血小板衍化生长因子（PDGF-BB）、结缔组织生长因子（CTGF）的含量，qRT-PCR和WesternBlot法检测TGF-β、Ⅰ型胶原蛋白（CollagenⅠ）和基质金属蛋白酶-2（MMP-2）的表达水平。结果：Masson染色显示木犀草素干预后瘢痕组织中胶原沉积减少，胶原纤维致密且排列规则。与生理盐水对照组相比，药物干预组瘢痕组织中TGF-β、PDGF-BB、CTGF的表达明显减少，差异有统计学意义（P

[关键词]木犀草素;增生性瘢痕;兔耳模型;机制研究

[]R619+.6??[文献标志码]A??[]1008-6455（2021）08-0091-04

PreliminaryStudyontheInhibitoryMechanismofLuteolinonHypertrophicScarinRabbitEar

LITai-ping，XIAOHong

（Neuro-PsychiatricInstitute，AffiliatedBrainHospital，NanjingMedicalUniversity，Nanjing210029，Jiangsu，China）

Abstract：Objective?Torevealtheinhibitoryeffectofluteolinanditsmechanismonhypertrophicscarformationinrabbitears.Methods?9NewZealandrabbitswererandomlydividedinto3groups：theblankgroup，thenormalsalinecontrolgroupandthedruginterventiongroup，with3rabbitsineachgroup.Hypertrophicscarmodelwasestablishedinrabbitearsinthenormalsalinecontrolgroupandthedruginterventiongroup.Beforeandfortydaysaftertreatment，thepathologicalchangesofthescartissueswereexamined，andthecollagenfibershyperplasiawereobservedwithMassonstaining.TheexpressionlevelsofTGF-β，PDGF-BB，CTGFwerequantifiedbyELISA.Inaddition，TGF-β，CollagenⅠandMMP-2wereseparatelydetectedbyquantitativereal-timePCR（qRT-PCR）assayandWesternBlot.Results?Massonstainingshowedthatcollagendepositionwassignificantlyreducedafterluteolintreatment，collagenfiberswerealsodenseandarrangedregularly.ELISAresultsindicatedthattheexpressionlevelsofTGF-β，PDGF-BBandCTGFwereobviouslyreducedinthedruginterventiongroupcomparedtothethenormalsalinecontrolgroup，thedifferenceswerestatisticallysignificant（P

Keywords：lute