BRCA1 BRCA2在结直肠息肉和癌中的表达及临床意义 .pdfVIP

BRCA1BRCA2在结直肠息肉和癌中的表达及临床意义

陈海滨;张国志;李曙光;王长友

【摘要】Objective:Todetectmembersofthefamilyofhomologousrepair

genesBRCA1andBRCA2expressionincolorectalpolypsandcancer,to

exploreitsrelationshipwiththeclinicalpathologicalfeatures.Method:By

usingimmunohistochemicalSPmethoddetected27casesofnormal

colorectalmucosa,30casesofcolorectalhyperplasticpolyp,33casesof

colorectaladenomassexpolyp,60casesofcolorectaladenocar-cinoma

tissueproteinBRCA1andBRCA2proteinexpression,analysedthephysical

indexandthecorrelationwithcolorectalpolypsandcancer.Result:BRCA1

andBRCA2innormalcolorectalmucosa,colorectalhy-perplasticpolyp,

colorectaladenomaspolypsandcolorectalcancerexpressionrate

increasedgraduallyintheorganization,andhadsignificantdifference(P

0.05),theexpressionincolorectalcancersuchassex,age,degreeof

differentiation,Ducksstagehadnostatisticaldifference(P0.05),bothin

thetissueofcolorectalcancerexpressionlevelsignificantlypositively

correlated(r=0.295,P0.05).Conclusion:ExpressionofBRCA1andBRCA2

arepositivelycorrelated,bothmayparticipateintheoccurrenceandde-

velopmentofcolorectalpolypsandcancer.Jointdetectionofcolorectal

lesionsintheorganizationtheexpres-sionofBRCA1proteinandBRCA2

proteinlevelsforearlydetectionofcolorectalcancer.%目的：检测同源修复

基因家族成员BRCA1、BRCA2在结直肠息肉和癌中的表达，探讨其与临床病理特

征的关系。方法：利用免疫组化SP法分别检测27例正常结直肠粘膜、30例结直

肠增生性息肉、33例结直肠腺瘤性息肉、60例结直肠腺癌组织中BRCA1、

BRCA2的表达水平。结果：BRCA1、BRCA2在正常结直肠粘膜、结直肠增生性

息肉、结直肠腺瘤性息肉、结直肠癌组织中的表达率逐渐增高，差异有显著性（P

＜0．05），二者的表达与结直肠癌中的性别、年龄、分化程度、Ducks分期等均

无统计学差异（P＞0．05）；二者在结直肠癌组织中的表达水平呈正相关（r＝

0．295，P＜0．05）。结论：BRCA1、BRCA2表达呈正相关；二者可能参与结

直肠癌的发生、发展；联合检测结直肠病变组织中的BRCA1蛋白、BRCA2蛋白

的表达水平有助于大肠癌的早期发现。

【期刊名称】《河北医学》

【年(卷),期】2015(000)005

【总页数】5页(P763-767)

【关键词】结直肠增生性息肉;结直肠腺瘤性息肉;结直肠