精氨酸酶-1在肝细胞癌中的低表达及其临床意义 .pdfVIP

精氨酸酶-1在肝细胞癌中的低表达及其临床意义

顾春燕;肖锋;钱铮;邵建国;秦刚;陈莉

【摘要】Backgroundandpurpose:Arginase-1(Arg-1)isanenzyme

involvedintheureacycle.Researchhasshownthatchangedexpressionof

Arg-1playsanimportantroleinthecellularmetabolismandgrowth.The

purposeofthisresearchwastoinvestigatetheexpressionofArg-1in

hepatocellularcarcinoma(HCC)andtoanalyzeitscorrelationwith

clinicopathologicalfeatures.Methods:TheexpressionofArg-1proteinand

mRNAin31samplesofHCC,paracancerouslivertissuesand12samplesof

normalliverwasdetectedbyWesternblotandreversetranscription-

polymerasechainreaction(RT-PCR).TheexpressionprofilesofArg-1at

proteinlevelin158samplesofHCCandparacancerouslivertissueswere

detectedwithhigh-throughputtissuemicroarraytechniqueand

immunohistochemistry.TherelationshipsbetweenArg-1expressionand

clinicopathologicalfeatureswerealsoanalyzed.Results:Theexpressionof

Arg-1mRNAandproteinwassigniifcantlydecreasedinHCCcompared

withtheparacancerouslivertissuesandnormallivertissues(F=57.83,

160.89;allP0.01).TheexpressionofArg-1inHCCwasrelatedto

differentiationdegree(F=10.41,30.03;allP0.01).Andwithtumor

differentiationdecreased,theexpressionleveldown-regulated.

ImmunohistochemistryrevealedthatArg-1proteinwasmainlylocatedin

thecytoplasmandnuclear.TheexpressionratesofArg-1were88%,

98.7%and100%inHCC,paracanceroustissuesandnormallivertissues,

respectively.StatisticalanalysisrevealedthatArg-1proteinstainingrate

wassigniifcantlylowerintumortissuesthanthatinparacanceroustissues

(χ2=14.7416,P0.01)andnormallivertissues(χ2=4.1415,P0.05).The

Arg-1expressionwascorrelatedwithdifferentiationdegreeofHCC,

vascularinvasionandrecurrenceafteroperation(χ2=22.8459,10.2639,

10.6368respectively;allP0.05),butnotcorrelatedwithage,gender,the

hepatitisBvirus,thelev