TACEF-基于酶标仪测定水中氨氮的试验方法及编制说明.pdf

ICS

T/ACEF20

Testmethodfordeterminationofammonianitrogeninwaterbasedonenzyme

marker

20--20--

中华环保联合会

I

11

21

31

42

52

62

74

84

95

106

116

127

138

148

1:(GB/T1.1-2000)

(SL1-2002)

:

I

1

N

200μl960.012mg/L0.10

mg/L2.0mg/LN

2

HJ535

GB17378.33

HJ91.1

HJ91.2

HJ164

HJ442.3

3

3.1ammonianitrogen

NHNH+

34

3.2microplatefluorimetricassay

1

4

4.1

4.2

420nm

5

5.1-

5.210~32

-

5.3

6

10MΩ·cm

6.1MgO

500

6.2(HCl)=1.18g/ml

6.3

2

6.3.1--HgCl2-KI-KOH

15.0gKOH50ml

5.0gKI10ml2.50gHgCl2

100

ml24h

1

6.3.2--HgI2-KI-NaOH

16.0gNaOH50ml

7.0gKI10.0gHgI2

50ml100ml

1

6.4=500g/L

50.0gKNaC4H6O64H2O100ml

100ml

6.5=3.5g/L

3.5gNa2S2O31000ml

6.6=100g/L

10.0gZnSO47H2O100ml

6.7=250g/L

25g100ml

6.8c(NaOH)=1mol/L

4g100