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CELLULARCARDIACELECTROPHYSIOLOGICALTECHNIQUESNORBERTJOST,PhD
ElectricalmodelofthemembraneStandardintracellularmicroelectrodetechniqueVoltageclamptechniquePatchclamptechnique
G=1/R
Ohm’slawIonchannelmodel
CurrentclampVoltageclamp
IntracellularmicroelectrodetechniqueReRinRin=1012Ohm
-0.2?m01添加标题Ag/AgClMKCl02添加标题Re~10-40MOhm03添加标题
ThesetupOrganbathd:stimulatingelectrodee:microelectroder:referentelectrodeP:preparationcomputerA/Dingerl?amplifierDetectedsignalPder100ms50mV0mV
drug20-60minmin0mVRP90%50%Wash-outPre-incubationAPAAPD90100msminVmaxAPD5020mV
TwomicroelectrodevoltageclampvoltagecommandholdingpotentialtestpotentialThemacroscopicsodiumcurrent
Thevoltage-clampcircuitvoltagecommandamplifierCurrentmeasurevoltagemeasurefollowupamplifier
Patch-clamp:thespecialcaseofthevoltageclamp
Cell单击此处添加大标题内容Patch-clamp:thespecialcaseofthevoltageclamp(1)Suckasmallpieceofmembraneontothetipofaglassmicropipette(~1μmindiameter)
Patch-clamp:thespecialcaseofthevoltageclamp(2)“Gigaohm-seal”CellR1GOhm
Cell单击此处添加大标题内容(3)Sensevoltagehere,insidetheelectrode,andusevoltageclamptokeepitconstant.Patch-clamp:thespecialcaseofthevoltageclamp
closedopenCell++Patch-clamp:thespecialcaseofthevoltageclamp(3)Sensevoltagehere,insidetheelectrode,andusevoltageclamptokeepitconstant.
closed openopen单击此处添加标题Cell单击此处添加标题(3)Turnontheaimedpotentialtheinsidepartofthepipetteandkeepitconstantlybyapplyingthevoltageclamptechnique.单击此处添加标题Patch-clamp:thespecialcaseofthevoltageclamp单击此处添加标题
voltagecommand10msecPropertiesofindividualvoltage-dependentsodiumchannels
Individualchannelsareeitheropenorclosed(nopartialopenings)Propertiesofindividualvoltage-dependentsodiumchannelsPART01
Individualchannelsareeitheropenorclosed(nopartialopenings)Eachchannelopeningisonlyabr
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