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功能基因组学
FunctionalGenomics;总结:以水稻为模式研究基因功能方法;Genesareclonedbasedon;actin1promoter;;;Wt 12-1 12-2
1.5%NaCl30days;Promotertrapping;Promotertrappinginrice;;;;;(Day);Genesareclonedbasedon;TheprincipleofMutMap;Pseudomonasaeruginosa
(5,000);GENENUMBER:
WhatIfThereAreOnly30,000HumanGenes?;HumanBeing Boeing767
30,000 200,000;OrganizationofGeneinGenome;Thinkingaboutgeneregulation;Atypicaldifferentiatedmammaliancellmakesabout10,000proteinsfromapproximately60,000genes.
Mostofthesearehousekeepingproteins
neededtomaintainallcelltypes.
Certainproteinscanonlybedetectedinspecificcelltypes.
Howisgeneexpressionregulated?;Regulationofgeneexpression;Euchromatinandheterochromatin
denselystainingobjectvisibleinsidethenucleusoffemalemammaliancells);EngineeringQuantitativeTraitVariationforCropImprovementbyGenomeEditing;课题组定位;;Photosynthesisisthefinalyielddeterminingfactor;Photosynthesisisthefinalyielddeterminingfactor;Sucrose;CO2fixationinhigherplants;InorganicPyrophosphatase(PPase);Plantsgrowthwasstunted;gdcsPpromoterexclusivelyexpressedinvasculartissuesinriceplants;GUSexpressionwasdevelopmentallycontrolledbygdcsPpromoterinleaves;Basepart;;GUSactivitywasdecreaseddramaticallywithseedmaturation;actin1promoter;Prerequisite1---anefficientricetransformationsystem;actin1promoter;TheT-DNAtaggingmutantpopulation;TheT1seedsfor25,000transformantswereobtained,andallT1werefinishedforphenotypinganalysisinthefield.
(database).;Anumberofmutantswithalteredagronomictraits;Elongateduppermostinternode(eui);;;PhenotypicanalysisofT1T-DNAmutantpopulation;Revolutionaryeventforriceproduction;Problemofhybridriceseedproduction
Panicleenclosureisatypicalphenotypeofalmostallmale-sterile(MS)lines.
Tosolvethisproblem,largeamountofexogenousGAwasappliedtoinducepanicleexsertion.
However,GAapplicat
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