流通卷20113社会杂志064smc-r1 let7 writing template064SMC R1 LET7写模板.pdfVIP

CirculationJournalORIGINALARTICLE

OfficialJournaloftheJapaneseCirculationSociety

VascularMedicine

VascularSmoothMuscleCellProliferationIsInfluenced

bylet-7dMicroRNAandItsInteractionWithKRAS

Man-LiYu,MD;Jia-FengWang,MD;Guo-KunWang,MD;Xiao-HuaYou,MD;

Xian-XianZhao,MD;QingJing,MD;Yong-WenQin,MD

Background:SeveralmicroRNAs(miRNAs)havebeenreportedtoregulatecardiovascularbiologicalandpath-

ologicalprocessesthroughinhibitingthetranslationofcertainRNAtranscripts.However,littleisknownaboutthe

associationbetweenmiRNAsandvascularsmoothmusclecell(VSMC)proliferation.Theaimwastoinvestigate

theroleofmiRNAsinVSMCgrowthandthepotentialmechanism.

MethodsandResults:PrimaryVSMCswereisolatedfromthemediallayerofthethoracicaortaobtainedfrom

spontaneouslyhypertensiverats(SHR)andWistarKyotorats(WKY).miRNAmicroarrayswereusedtoanalyze

thedifferenceinmiRNAexpressionbetweenVSMCsofSHRandWKYratsandwerevalidatedusingTaqMan

real-timePCR.Ofthepotentiallyrelatedgenesundertheinfluenceoflet-7didentifiedthroughliteraturesearch,

KRASwasverifiedbywesternblotandfunctionallyanalyzedusingmiRNAmimicstransfectionandanalysisof

transfectantsbycellenumerationwasmadeusingCCK-8andflowcytometricanalysisofcellcycleprogression.

let-7d-transfectedVSMCsfromSHR,WKYandhumancoronaryarteriepressedsignificantlyloweramountsof

KRASprotein,displayedreducedcellgrowthandledtoagreat