dna靶向引导核酸酶特异性crisp基因改造nihms.pdfVIP

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NatBiotechnol.2013September;31(9):827–832.doi:10.1038/nbt.2647.

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utargetingspecificityofRNA-guidedCas9nucleases

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r1,2,3,91,2,91,21,2,3

PatrickDHsu,DavidAScott,JoshuaAWeinstein,FAnnRan,Silvana

M1,21,4,51,267

aKonermann,VineetaAgarwala,YinqingLi,EliJFine,XuebingWu,Ophir

n1,26861,2

Shalem,ThomasJCradick,LucianoAMarraffini,GangBao,andFengZhang

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cBroadInstituteofMITandHarvard,Cambridge,Massachusetts,USA

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p2McGovernInstituteforBrainResearch,DepartmentofBrainandCognitiveSciences,

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DepartmentofBiologicalEngineering,MassachusettsInstituteofTechnology,Cambridge,

Massachusetts,USA

3DepartmentofMolecularandCellularBiology,HarvardUniversity,Cambridge,Massachusetts,

USA

4PrograminBiophysics,HarvardUniversity,Cambridge,Massachusetts,USA

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HHarvard-MITDivisionofHealthSciencesandTechnology,MIT,Cambridge,MA02139,USA

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P6DepartmentofBiomedicalEngineering,GeorgiaInstituteofTechnologyandEmoryUniversity,

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