高效质粒提取套件操作手册.pdfVIP

Manual

Productname:MaxismidPurificationKit

Cat#:EFX-100,EFX-200,EFX-300

Description

AnE.colicultureisharvestedbycentrifuionandtreatedtoamodifiedalkaline-SDSlysis

procedure.Followinglysateclearing,isselectivelyadsorbedtotheicparticinthe

presenceofhighsaltsandalcohol.Contaminantsareremovedbytwowashingsteps.Thebound

isthenelutedandsubjectedtoisopropanolprecipitationasafinalpolishingstep.

Therecoveredproductisofhigh-qualityandpredominantlysupercoiledsmidthatis

readyforimmediateuseindownstreamapplicationssuchasrestrictiondigestion,liion,

sequencing,PCR,transformationandtransfection.

Features

•Fast(prepin30minutes)

•Highquality(supercoiledsmidreadyforallapplications)

•Highyield(upto1mgperprep*)

•Invivostudyready(endotoxinfree**)

•Nophenol/chloroformextractionoralcoholprecipitationrequired

*Actualyieldsmayvarybasedonthebacterialstrain,smidandcultureused.

**MCLAB’sendotoxinfreestandardis0.1EU/µgsmid.

EFX-100components

BufferP150ml

BufferP250ml

BufferT350ml

ETRBindingBuffer75ml

icBeads10ml

Wash1Buffer200ml

TFBuffer10ml

FilterSyringe10pc

RNaseA5mg

Endotoxin-FreeTE30ml

手动的

产品名称：Maxi质粒纯化试剂盒

目录号：EFX‑100、EFX‑200、EFX‑300

描述

通过离心收获大肠杆菌培养物，并采用改良的碱性SDS裂解程序进行处理。裂解液澄清

后，在高盐和存在下选择性吸附到磁性颗粒上。通过两个洗涤步骤去除污染物。然

后洗脱结合的并进行异丙醇沉淀作为最终的精制步骤。

回收的产品具有高质量且主要是超螺旋的质粒，可立即用于，例如限制

性、连接、、PCR、转化和