荧光漂白恢复实验流程.pdfVIP

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  • 2026-03-05 发布于河南
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荧光漂白恢复实验流程

FluorescenceRecoveryAfterPhotobleaching(FRAP)

ExperimentProtocol.

Materials:

Cellsofinterestexpressingafluorescently-tagged

protein.

Fluorescencemicroscopewithphotobleaching

capabilities.

Photobleachinglaser.

Fluorescencerecoverycurveanalysissoftware.

Procedure:

1.Preparethecells:Growandpreparethecellsof

interestaccordingtostandardprotocols.

2.Mountthecellsonamicroscopeslide:Useaglass

orquartzcoversliptomountthecellsonthemicroscope

slide.

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