蛋白质基本性质的分析秋.pptxVIP

1第六章蛋白质基本性质旳分析

2023-11-17

2本章旳主要内容一、蛋白质理化性质旳分析二、酶切图谱三、亲疏水性分析四、抗原性分析五、表面分析六、柔性分析七、二级构造预测

3分析界面TheAssayDocumentisthefilecreatedandusedbyPROTEANtoexamineandelucidateproteinsequences.Itisoftenreferredtoassimplyanassay.Theassayiscomposedoffiveprincipleparts:thestatusarea,thepalettetools,theMethodCurtain,theassaysurfaceandtheLegendCurtain.Clickintheassaygraphicbelowformoreoneachpart.MethodcurtainAnalysissurfaceLegendcurtain

4Twoselection

5Addaknownfeature

6Joinafeature

7UsingtheMicroscopeAMicroscopeisprovidedforsituationswhereyouwanttoseethesequenceresponsibleforamethodsresult.Thisletsyoustayzoomedoutfortheoverallbigpicture,butstillseecriticalresiduesinaregion.ClicktheMicroscopepalettetooltoactivateitsdisplaywindow.Youhaveyourchoiceofdisplayingthesequenceaschemicalformulaorspace-fillingmodels.JustclicktheappropriatebuttonintheupperleftcorneroftheMicroscopewindow(asshowninthefollowingfigures).ThearrowboxinthelowerleftcornercontrolsthesizeoftheaminoacidsequencecharactersandtheverticalsizeoftheMicroscopewindow.ThearrowboxinthelowerrightcornercontrolsthehorizontalsizeoftheMicroscopewindow.Drageitherboxtocontrolthesizeofthemini-window.Asthecursorspositionchangesovertheassaysurface,PROTEANupdatestheMicroscopewindowtodisplayresiduesunderneath.YoucanexaminetheentiresequencebychoosingeitherLinearSpaceFillorChemicalFormulafromtheModelStructuresubmenu(undertheAnalysismenu).

8TabularDataThefirstcolumnshowsanaminoacid,theseconditspositionandtheremainingcolumnsshowthevalueassignedforeachresidueatthegivenposition.Double-clickanyvalueorcolumntoshowtheparameterwindowforthemethodinquestion.ChangingtheorderofplotsontheassayrearrangesthecolumnsummariesontheTabularDatawindow.

9Modelstructure