如何探讨中药有效成分对基因调控的影响专家讲座.pptx

怎样探討中藥有效成份對

基因調控影響

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StimulationInflammatoryproteinSignaltransductionTranscriptionfactorProteinkinasephosphorylationProtein-DNAbindingProteinmRNAAP-1NF-?BAP-1NF-?BEgr-1MCP-1TF(WesternBlot)*(EMSA)(NorthernBlot)(ELISA)(ex.ERK,P38,JNK)(ex.AP-1,NF-?B,Egr-1)ReceptorGeneRegulationMembraneNuclearex.TNF,IL-1,MCP-1,ICAM-1ex.cytokine,LPS,stressCM98/03/07/94第2页

ElectrophoreticMobilityShiftAssay(EMSA)研究DNA結合蛋白與其相關結合序列相互作用方法Protein/DNA(transcriptionfactor)/(promoterregion)第3页

EMSANuclearproteinDNAprobeNativegel(non-denaturingag