RP-HPLC法测定男宝胶囊中淫羊藿苷和丹皮酚的含量.doc

RP-HPLC法测定宝胶囊中 吴秀清李远科（ 1 四川省资阳药检所资阳 6413002. 成都中医药大学药学院, 成都 610075 ） 摘要 目的：RP-HPLC法同时测定宝胶囊中的含量。方法：高效液相色谱法测定批不同药厂生产的宝胶囊中的含量。色谱系统： C8 色谱柱（250 mm×4.6 mm，5 μm），柱温25℃，检测波长为 nm，流动相，流速：1.0 mL·min-1。结果：本法灵敏度高，重现性好，提取方法与色谱条件均较简单，能有效鉴别测定中的含量。结论：该方法操作简便、灵敏，结果准确可靠。可用于宝胶囊中的含量测定。 关键词：宝胶囊高效液相色谱法Determination of icariin and paeonol in Nan Bao Capsules by RP-HPLC Wu Xiu-qing1 , Li Yuan-ke1 , Liang Wei2, Huang Wei1 (1. Sichuan Ziyang Institute for Food and Drugs Control, Ziyang 641300; 2. Chengdu University of Traditional Chinese Medicine, Chengdu 610075) Abstract Objective: To develop a HPLC method for the determination and analysis of the icariin and paeonol within Nan Bao capsules. Method: 6 samples of Nan Bao capsules from different producers were collected. The content of icariin and paeonol were all determined by RP-HPLC method. The analysis was performed on a HPLC apparatus equipped with a C8 column (DiamonsilTM, 250 mm×4.6 mm，5 μm) at 25℃. The detection was carried out at a wavelength of 270 nm , the mobile phase consisted of Water(phase A)， Acetonitrile (phase B) with gradient elution with the flow rate of 1.0 mL ·min-1.Results: This method possessed desirable accuracy and reproducibility as well as simplicity. The extraction method and the conditions for HPLC were quite simple to perform. It was effective in distinguishing and determing icariin and paeonol in Nan Bao capsules. Conclusion: The method proved to be effective, accurate, and could be applied to quantitatively analyse icariin and paeonol in Nan Bao capsules. Key words: Nan Bao capsules; icariin; paeonol; HPLC 男宝胶囊[1]，由鹿茸海马阿胶牡丹皮淫羊藿驴肾黄芪壮阳补肾用于肾阳不足引起的性欲淡漠，阳痿滑泄，腰腿酸痛，肾囊湿冷，精神萎靡，食欲不振等症淫羊藿淫羊藿牡丹皮1 仪器与试药 仪器：高效液相色谱仪、泵、、、检测器工作站XS205DU十万分之一分析天平。 吴秀清对照品：（批号：-200312）、（批号：-200505）由中国药品生物制品检定所提供，供含量测定用。 试剂：甲醇为分，流动相为色谱纯 Corporation)，水为超纯水。样品：四川省资阳药检所。2 方法与结果 2.1 色谱条件 C8色谱柱（250 mm×4.6 mm，5 μm）；柱温：25；流动相：，；流速：1.0 mL?min-1；检测波长：nm。表1.Time/min 0 20 35 40 Mobile phase B/％ 20 35 60 90 2.2 溶液制备 2.2.1 供试品溶液　精密取宝胶囊内容物约g ，置于mL量瓶中，加入甲醇2