苦参方成分分析及配伍机制研究.doc

苦参方彭程，，胡晋红* (第二军医大学长海医院药学部，上海 200433) [摘要]目的：中医治疗过敏性皮肤病的传统方剂苦参方有效部位成进行定性定量分析，研究方剂配伍成份。方法：采用LC/MS、GC/MS技术确定苦参方的有效成分；采用HPLC色谱法对苦参方生物碱含量进行定量分析，考察方剂配伍后的成变化。结果：苦参方挥发油中的主要成有：胡薄荷酮、薄荷酮等，所含苦参生物碱主要有苦参碱（Mat）、氧化苦参碱（Omt）、槐果碱（Sop）、氧化槐果碱（Osp）；结论：。[关键词]苦参方；苦参；荆芥；苦参碱；氧化苦参碱； Composition Analysis And Transformation Mechanism Study Due To Compatibility Of The Recipe Of Kushen. PENG Cheng, HU Jin-hong*, ZHU Quan-gang , LIU Ji-yong, QIN Zhuo, GAO Li-hong, WANG Jing （Dept of Pharmacy, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China） [ABSTRACT] Objective：ualitative and quantitative analysis for effective components in a traditional anti-anaphylaxis Chinese medicine of Kushen was established, and the content’s change of the active composition was testified, which was due to compatibility between Sophora flavescens Ait. and Schizonepeta tenuifolia Briq.. Methods： The effective components confirmed by LC/MS and GC/MS. HPLC was utilized to quantitate the alkaloids, so as to investigate the change of active composition’s content. Results：Thecomponents of Schizonepeta tenuifolia Briq. volatile oil were l- menthone and (+)-pulegone, etc..The alkaloids extracting from the recipe included matrine, oxymatrine, sophocarpine and oxysophocarpine. The content of alkaloids changed because of compatibility, oxymatrine transformed to matrine and oxysophocarpine transformed to sophocarpine partly, the ingredients and content of volatile oil were different before and after compatibility. Conclusion：ingredients, content and transformation were identified, so the traditional recipe would be applied more definite, reasonable and scientific. [KEY WORDS] Recipe of Kushen；Sophora flavescens Ait.；Schizonepeta tenuifolia Briq.；Matrine；Oxymatrine；ophocarpine； Oxysophocarpine；Volatile oil；Assay；Compatibility；Transformation 苦参方由苦参、荆芥两味药组成，于《太平惠民和剂局方》，原方为治疗疥癣、湿疹和皮肤瘙痒的有效方剂。现代药理研究证明，苦参中苦参型生物碱和荆芥中荆芥挥发油均具有良好的调节免疫和抗过敏作用[][]。采用多种分析手段，确定了该方剂有效部位的具体成及含量，方剂配伍后发生成，使这一中药传统方剂更加明确、科学、合理。 1 仪器和