《人体显微形态学》3 In situ display of DNA and RNA in the cell.pptVIP

THE 3rd EXPERIMENT In situ display of DNA and RNA in the cell [Aims] To master the method of in situ display of DNA and RNA in the cell To learn the method of the blood smear, To know the process of fixation and dyeing. [ principles ] DNA and RNA could be dyed different colors by the solution mixed with methylgreen and pyronine because of the different polymeric degree. DNA is dyed to be green and blue by methylgreen for its high polymeric degree, whereas RNA is dyed to be red by pyronine for its low polymeric degree. [Contents and Manipulations] Preparing a smear of blood cells from the