丙酯草醚对不同类型油菜叶绿素荧光的影响.doc

  丙酯草醚对不同类型油菜叶绿素荧光的 影响# ** 5 10 15 20 25 30 35 40  摘要：通过新型油菜田除草剂丙酯草醚（ZJ0273）处理，研究其对抗性油菜品种甘蓝型油菜 浙双 758（Brassica napus cv. Zheshuang 758）和敏感性品种白菜型油菜小油菜（B. rapa cv. Xiaoyoucai）植株叶绿素荧光的影响。大田油菜用不同浓度的丙酯草醚（0、100、500 和 1000 mg/L）进行处理，并分别在处理 1、3、5、7 天后，进行叶绿素荧光测定。研究表明，随供 试植株叶片叶龄的增加和丙酯草醚处理浓度的上升，两种油菜叶片的叶绿素荧光参数最大光 电子产量（Fv/Fm）、非光化学粹灭（NPQ）、实际光电子产量（Y (II)）等受到的抑制作用 均逐步加剧，但甘蓝型油菜在处理 5 天后抑制作用显著缓解。通过电子传递速率（ETR）和 Y (II)的浓度抑制曲线，我们发现 500 mg/L ZJ0273 对甘蓝型油菜生长安全，但对白菜型油菜 仍有 20%左右的抑制效果。在除草剂处理下植株叶片的 Fv/Fm、NPQ、Y (II)和 ETR 等参数 的抑制率变化趋势，均能表现植株的药害程度以及反映不同品种油菜的抗性差异，故对这些 参数的研究有助于构建除草剂胁迫下植株药害和抗性检测系统。此外，研究还发现油菜植株 第二片完全展开叶能较好地反映植株所受药害程度的差异。 关键词：油菜；丙酯草醚；叶绿素荧光；抗性检测 中图分类号：S332 Differential chlorophyll fluorescence responses of two oilseed Brassica species to herbicide ZJ0273 JIN Zonglai1, CUI Peng2, XU Ling2, ZHOU Weijun2,3 (1. Wenzhou Agriculture Bureau, ZheJiang WenZhou 325000; 2. Department of Agronomy, Zhejiang University, HangZhou 310058; 3. Agricultural Experiment Station, Zhejiang University, HangZhou 310058) Abstract: As a newly developed post-emergence herbicide, propyl 4-(2-(4,6-dimethoxypyrimidin -2-yloxy)benzylamino)benzoate (ZJ0273) is recently becoming popular in China. To develop a resistance monitoring tests for the crops in a non-destructive, real-time and efficacious way, two cultivars of rapeseed Brassica napus cv. ZS 758 (resistant type) and B. rapa cv. Xiaoyoucai (susceptible type) were tested by a foliar spray of ZJ0273 at the rates of 100, 500 and 1000 mg/L. Our study revealed that Fv/Fm, NPQ and Y(II) were significantly decreased with the herbicide stress and leaf age increasing. However, the resistant B. napus species started to recover at 5 days after treatment. Moreover, the dosage-inhibiting curves of Y(II) and ETR suggested that the 500 mg/L was a safer dose for B. napus, which induced an approximately 20 % inhibition in B. rapa. These results showed that the use of Fv/Fm, NPQ, Y(II) and ETR inhibiting rate may be appropriate for herbicide phytotoxicity detector and also se