活性氧诱导腹水综合征肉鸡心肌细胞凋亡.doc

  活性氧诱导腹水综合征肉鸡心肌细胞凋亡# 席照房，杨世锦，柳东阳，吴黎明，赵靖，刘孝东，郭定宗** （华中农业大学动物医学院，武汉 430070） 5 10 15 20 25 30 35 40 摘要：科研学者普遍认为腹水肉鸡死于肺动脉高压引起的右心衰竭，然而引起右心衰的具体 机制一直未被完全理解。经检测，心肌细胞的凋亡能够引起心肌的衰竭，许多研究表明在人 和鼠的心脏病理模型中，活性氧有损伤并诱导心肌细胞凋亡的能力。本试验将重点检测肉鸡 腹水综合征发生发展过程中，活性氧在血清、血浆中的变化趋势，以及活性氧引起的脂质氧 化对肉鸡心脏及单个心肌细胞的影响。通过运用低温和缺氧的方法对快速生长的肉鸡，进行 肉鸡腹水综合征发病诱导，来建立腹水综合征肉鸡发病模型。并以相同处理条件下的低速生 长（70%限饲）肉鸡作为对照。试验结果证明，比单纯的低温和高速生长诱导腹水综合征来 讲，缺氧是更为有效的诱导方法。此外，在腹水综合征诱导组中血清、血浆中的活性氧的含 量比对照组有着显著的升高。且这种活性氧含量的明显上升，增加了体内脂质氧化水平，导 致了腹水肉鸡的心肌细胞的凋亡。试验结果显示，在腹水综合征发病过程中，肉鸡代谢紊乱 造成的血清、血浆中的活性物质升高而引起的心肌细胞凋亡是导致右心衰竭的主要原因。 关键词：活性氧(ROS); 腹水综合征；脂质氧化；凋亡 中图分类号：S856.5 ROS Induce Cardiomyocyte Apoptosis in Ascitic Broiler Chickens XI Zhaofang, YANG Shijin, LIU Dongyang, WU Liming, ZHAO Jing, LIU Xiaodong, GUO Dingzong (College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China 430070) Abstract: It is believed that ascitic broilers die of right heart failure caused by pulmonary hypertension, but the underlying mechanisms of right heart failure are unknown. Recent studies have shown that reactive oxygen species have the ability to damage heart cells. This study aimed to determine the changes of reactive oxygen species in serum and plasma, and the effect of this variation on myocardial cells during broiler ascites. We used hypoxia and a low-temperature method to induce broiler ascites in the fast-growing group. For controls, we treated a slow-growing group of broilers with 70% restricted feeding under the same circumstances as the fast-growing group. The results showed that hypoxia is a more effective and better way to induce broiler ascites than a low-temperature environment and high growth rate. In addition, reactive oxygen species levels were significantly increased in the fast-growing group compared with those in the slow-growing group. This significant increase in reactive oxygen species resulted in myocardial cell apoptosis in the fast-growing group. Our results suggest that cardiomyocyte apoptosis caused by increased reacti