辣椒碱预防大鼠营养性肥胖作用的研究.docVIP

  辣椒碱预防大鼠营养性肥胖作用的研究# * 5 10 15 20 25 30 35 40 45 摘要：目的：观察辣椒碱对超重大鼠体重、体脂含量及血糖血脂的影响，探讨辣椒碱预防营 养性肥胖的作用。方法：将 48 只大鼠随机分为正常对照组、模型组和辣椒碱低、中、高剂 量干预组，利用高糖高脂饲料诱导建立营养性超重大鼠模型，辣椒碱干预 4 周，观察体重、 内脏脂肪湿重的变化，采用酶法检测血清葡萄糖（GLU）、甘油三酯（TG）和总胆固醇（TC）， 选择性沉淀法检测低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）。结果： 辣椒碱各剂量组大鼠体重低于模型组；中剂量组内脏脂肪湿重低于模型组，高剂量组内脏脂 肪湿重、内脏脂肪/体重均低于模型组（P 0.01）；与模型组相比，各剂量组大鼠血清 TC、 LDL-C、TG、GLU 水平降低，而 HDL-C 水平升高（P 0.01），呈剂量-效应关系。且高剂 量组大鼠各项指标与正常对照组相比无差异。结论：辣椒碱可有效抑制超重大鼠体重的增加， 减少体脂聚集，调节血糖血脂水平，对营养性肥胖有显著的预防作用。 关键词：辣椒碱；预防；超重；营养性肥胖；大鼠 中图分类号：R153 Prophylactic Effect of Capsaicin on Alimentary Obesity of Rats QIN Hong, REN Guofeng, YUAN Quan (School of Public Health, Central South University, Changsha 410078) Abstract: Purpose: To observe the effects of Capsaicin on body fat, plasma lipids and glucose in overweight rats and investigate the prophylactic effect of Capsaicin on alimentary obesity. Methods: 48 rats were divided randomly into five groups: normal-control group (ND), model group (HD), low Capsaicin supplement group (HD+5), middle Capsaicin supplement group (HD+10) and high Capsaicin supplement group (HD+20). The rats were fed high fat and carbohydrate diet to induce overweight, and then treated with Capsaicin for four weeks. We observed the changes of body weight and wet weight of the visceral fat of the rats. The plasma glucose (GLU), total triglycerides (TG), total cholesterol (TC) were assayed with enzymic method, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were assayed with selective precipitation method. Results: The body weight of Capsaicin treated groups were lower than the HD group. The wet weight of visceral fat of HD+10 group was lower than the HD group, and the wet weight of visceral fat and the visceral fat/body weight of HD+20 were lower than the HD group (P 0.01). Compared with the HD group, the plasma TC, LDL-C, TG and GLU were reduced in Capsaicin treated groups, while the HDL-C in these three groups were increased (P 0.01), a