基因专业毕业论文--鹅白细胞介素-1的克隆.docVIP

摘 要 白细胞介素-1( IL-1 ) ，，。 ILIL-1α和IL-1β。IL-1具有广泛的免疫调节作用，并有致热和介导炎症的作用，，，它的生物学功能是通过与相应高亲和力受体结合而介导的。。。，，，IL-1作用无明显的种属特异性，人IL-1可作用于小鼠源性的细胞。，，，: 白细胞介素-1, 转化 , 克隆  Abstract Interleukin-1(IL-1), molecular weight 17kD, is a important cell factor that participates in immune reactions and inflammation processes. IL-1 has two molecular structures: IL-1αand IL-1β.IL-1 has comprehensive immunoregulation function, and has the function of producing heat and mediating inflammation. In addition, it has an important impact on the metabolism of bones and the propagation of animals, its function of biology mediates by combining with high appetency receptors. Generally speaking, it mediates non-specific immune reactions and regulates functions of immune system. According to recent studies, IL-1 and its receptors were also found in the central nervous system (CNS). IL-1 acting as a neurotransmitter, neuromodulator/growth factor,involves in normal physiological processes and pathogenesis of diseases in the CNS. Further more, IL-1 activates multiplicate phosphatide enzymes on cell membrane and plasm, and produces many kinds of messenger substances by the functions of some regulate proteins after it combines with its specific receptors. IL-1’s functions have no obvious differences in different genera, human IL-1 could effect on the cell that come from rat. This experiment enlarged the production of RT-PCR from total RNA in Gooses’ blood corpuscles, and got the gene of Goose IL-1. We reclaimed the production by using DNA gel extraction kit, and connected it with pMD18―T vector., transformed it to E.coli JM-109 and got the cloning production of Goose IL-1.It establishes the foundation of researching gene expression, biological activation and the application of IL-1 of Gooses. Key words: Interleukin-1, transform, clone 目 录 摘要 ⅠAbstract Ⅱ 1前言 1 1.1白细胞介素-1的结构和功能 1 1.2国内外IL-1的研究进展 3 1.3本实验的研究意义 5 2实验材料及试剂 6 2.1实验材料 6 2.2主要试剂 6 2.3实验仪器 8 3实验方法 9 3.1 IL-1基因的PCR扩增 9 3.2 PCR产物的回收