毕业论文-金属离子的探针检测.docVIP

摘　　要 金属离子是化学、生命科学、环境科学和医学等许多科学领域研究的重要对象，对溶液中金属离子的识别和检测是分析化学的主要任务之一。荧光分子探针检测法不仅简便，而且在高灵敏度、选择性、时间分辨、实时原位检测方面均有突出优点。因此在传统的受体分子上按照荧光分子传感器设计原理连接荧光团构造的超分子荧光传感器用于识别金属离子的研究受到越来越广泛的关注。 本论文主要工作是对已合成的荧光探针化合物利用紫外可见分光光度计和荧光分光光度计进行检测。本研究进行了多个不同探针对不同金属离子的检测，其中有两个比较成功。一个是对铜离子有高选择性的探针A对水中铜离子检测，在对检测液进行3D扫描后得出探针A的检测波长，即探针A检测铜离子的激发波长EX=550nm，发射波长EM=590nm。在对不同浓度的铜离子的荧光扫描中得出探针检测铜离子的标准曲线，其线性方程为y=1.761x+12.4。从标准曲线 中得出该探针A的检测限为6.345μM／L。该探针检测限低，具有良好的选择性，无其他金属干扰。是一种方便快捷的检测方法。另一个是对汞离子具有高选择性的探针B对水中汞离子的检测，在对检测液进行3D扫描后得出探针B的检测波长，即探针B检测汞离子的激发波长EX=570nm，发射波长 EM=594nm。在对不同浓度的汞离子的荧光扫描中得出探针B检测汞离子的标准曲线，其线性方程为y=1871.8x―181.5。从标准曲线中得出探针B的检测限为0.239μM／L，在对其进行不同PH检测中得出检测液的适宜PH值为6—8 。在此PH值响应值较高，检测结果较理想。还得出该探针具有良好的选择性，我其他金属干扰。探针B的优点是水溶性好，完全水溶，使得在实际应用中可直接用水做缓冲溶剂，使检测更加方便快捷。 关键词：金属离子；荧光探针；Cu2+；Hg2+ Fluorescent chemosensors for selective detection of metal ions in natural water Abstract The analysis and detection of the transition metal ions is currently of significant importance for chemistry, as they are closed with biology, environment and clinic. The method of fluorescence is not only simple but also can realize space, real time, high sensitive and selective. So the fluorescence supramolecular sensors, which are composed of traditional receptors connecting fluorophores, to metal ions are attracting many people’s attentions. In this paper, our main work is to test the fluorescent probe A that have already synthesized by UV-vis spectrophotometer and fluorescence spectrophotometer. During the research progress, we test several different probes, in which the following two is the best. The first one is a high selective probe of copper ions in a CH3CN/H2O solution. We got the Excitation wavelength 550nm and the emission wavelength 590nm by the 3D scanning. We also tested different concentrations of copper ions with the fluorescent probe to get the standard curve y=1.761x+12.4. And the gainod the detection limit to 6.345 from the standard curve. This fluorescent probe has advantages of low detection limit, good