甲状腺激素受体在妊娠合并甲亢母鼠心脏中的差异表达毕业论文.doc

甲状腺激素受体在妊娠合并甲亢母鼠心脏中的差异表达 内容摘要 [摘要] 目的：通过对甲状腺功能亢进症（简称甲亢，Hyperthyroidsm,TH）母鼠心脏、肝、肾、脑组织中的甲状腺激素受体的检测，来讨论上述因子在个脏器中的差异表达。 方法：通过给予甲状腺素片连续灌胃的方法制作甲亢母鼠模型，期间所有母鼠均自由进食水，在孕16天、18天、20天分批宰杀实验组（分别为A2-3、B3-2、A2-2、B2-1组）和对照组（C2-2、C5-1、D4-2、D2-2组），提取RNA做PCR，电泳后观察GAPDH、Tα1、Tα2、Tβ1的表达。 结果：通过对灰度值的分析，对照组的Tα1、Tα2、Tβ1的灰度值比GAPDH的灰度值低，而实验组有的Tα1、Tα2、Tβ1的灰度值比GAPDH的灰度值高；实验组的Tα1、Tα2、Tβ1的灰度值比对照组的Tα1、Tα2、Tβ1的灰度值高。说明，甲状腺激素受体在妊娠合并甲亢母鼠心脏中的表达比正常妊娠合并母鼠心脏中的表达高。 [关键词]：甲状腺功能亢进症; 甲状腺激素受体;GAPDH; Tα1;Tα2;Tβ1 Thyroid hormone receptor in pregnancy with hyperthyroidism. The differentially expressed females heart ABSTRACT Objective: to thyroid function through the hyperthyroidism (hereinafter referred to as overbearing, Hyperthyroidsm, TH) females heart, liver, kidney, in the brain of thyroid hormone receptor detection, to discuss the above factors of differences in a visceral expression. Methods: by giving thyroxine piece continuous irrigation method of making hyperthyroidism stomach during the mother, the mother are all model of water, free food in pregnant 16 days, 18 days, 20 days respectively the partial killed three, B3 - A2-2 and A2-2, B2-1 group) and the control group (C2-2 and C5-1, the D4-2, D2-2 group), extraction RNA polymerase chain reaction (PCR), electrophoresis do after GAPDH, T alpha 1 observe, T alpha 2, T beta 1 expression. Results: according to the analysis, the control group grey value of T alpha 1, T alpha 2, T beta 1 gray value GAPDH gray value than low, experimental some T alpha 1, T alpha 2, T beta 1 gray value GAPDH gray value than high; The T alpha 1, experimental T alpha 2, T beta 1 gray value than the control group T alpha 1, T alpha 2, T beta 1 gray value high. Instructions, thyroid hormone receptor in pregnancy with overbearing mother than normal heart the expression of the expression mother pregnancy with high heart. Keywords: thyroid function hyperthyroidism; Thyroid hormone receptor; GAPDH; T alpha 1; T alpha 2; T beta 1 前言 甲状腺功能亢进症(简称甲亢，Hyper