β榄香烯对胃癌细胞Akt 通路活化和凋亡相关蛋白表达的影响.pdfVIP

现代肿瘤医学 2012 年 3 月 第 20 卷第 3 期 MODERN ONCOLOGY ，Mar. 2012 ，VOI. 20 ，NO. 03 ·45 1 · β － 榄香烯对胃癌细胞 Akt 通路活化和凋亡相关蛋白表达的影响 1 1 1 1 2 1 1 1 ， ， ， ， ， ， ， 张 晔 赵明芳 刘云鹏 曲秀娟 杨 向红 侯科佐 滕 月娥 张敬 东 The effects of β － elemene on the activation of Akt and the expressions of apoptosis － re- lated proteins in gastric cancer ZHANG Ye1 ，ZHAO Mingfang1 ，LIU Yunpeng1 ，QU Xiujuan1 ，YANG Xianghong2 ，HOU Kezuo1 ，TENG Yuee1 ， ZHANG Jingdong1 1 Dep artment of Medical Oncology ，the First Hosp ital ，China Medical University ，Liaoning Shenyang 110001，China; 2 Dep artment of Pathology ，Shengj ing Hosp ital ，China Medical University ，Liaoning Shenyang 110004 ，China． 【Abstract 】 Objective: To explore the effects of β － elemene on the activation of Akt and the expressions of apopto- sis － related proteins of human gastric cancer BGC823 cells． Methods: Cell proliferation was measured with MTT assay ． The expressions of proteins were detected by Western blot ． All experimental data were analyzed by SPSS ( 13． 0) ． Results: β － Elemene coule inhibited BGC823 cell viability and the concertration of inhibited cell viability ( IC50 ) for 24h was 46． 56 μg / ml． After treatment with 50 and 200 μg / ml β － elemene for 24h ，the rates of cell apop- tosis were 12 ． 33 and 42 ． 59 ，respectively． β － Elemene decreased the ratio of Bcl － 2 / Bax expressions and caused the cleavage of PARP． After treated 24 h ， － elemene could obviously decrease the expressions of phosphorylated β Akt in BGC823 cells． Conclusion: β － Elemene inhibited the activation of Akt signaling pathway ，which consequent- ly down － regulated the value of Bcl －