茶籽饼粕中茶皂素的提取及优化工艺.docVIP

学生毕业论文 题 目：茶籽饼粕中茶皂素的提取及优化工艺 姓 名： 宋雪琴 学 号： 201012020123 系 别： 应用化学系 班 级： 生化1011 指导教师： 罗跃中老师 2012 年 12月 摘 要 茶籽榨油后所得的茶籽饼中含有一定的茶皂素，而从茶籽饼中提取茶皂素并使之综合利用的问题变得尤为重要。由于茶皂素其味苦而辣，从而影响茶籽饼的进一步回收与利用。茶籽饼中茶皂素的利用也就成了关键技术问题之一。我们用微波提取法从提取剂、提取浓度、提取次数、料液比等四个方面对茶籽饼中茶皂素的提取及含量测定做了进一步的探讨与研究。再利用紫外分光光度计进行扫描，结合提取出的茶皂素提取液，再经香草醛—硫酸显色法，得出茶皂素在470nm处出现最大吸收峰，即确定茶皂素的最大吸收波长为470nm。 用茶皂素标准样，经香草醛-硫酸显色方法，绘制茶皂素定量的标准曲线，然后通过单因素试验研究了提取剂、提取浓度、提取次数、料液比对茶皂素提取率的影响，通过正交试验确定了茶皂素的最佳工艺参数：提取剂为乙醇、乙醇浓度为10%、提取次数为两次，茶皂素的提取率最高。其提取率为3.62%。 关键词：茶籽饼、茶皂素、提取、含量测定、单因素试验、正交试验 Title In tea-seed Cake Tea Saponin Extraction And Determination Research Abstract Tea seed oil from the tea seed cake contains a certain amount of tea saponin, and extraction of tea Saponin from tea seed cake and comprehensive utilization of issue has become particularly important. Due to its bitter and spicy of tea saponin, which further recovery and utilization of tea seed cake. Utilization of tea saponin in Camellia cake has become one of the key technical issues. We with microwave extraction law from extraction agent, and extraction concentration, and extraction number, and material liquid than, four a area on tea seed pie in the tea saponin of extraction and the content determination do has further of discussion and research, uses purple outside points light photometer for scan, combined extraction out of rough tea saponin and tea saponin standard products, by vanillin-ethanol solution explicit color, concluded that tea saponin in 450nm Department appears maximum absorption peak, that determines tea saponin of maximum absorption wavelength for 450nm. Tea saponin standard sample, by vanillin-ethanol explicit color method, draws tea saponin quantitative of standard curve, then through single factors test research has extraction agent, and extraction concentration, and extraction number, and material liquid than on tea sa