HPLC梯度洗脱法测定核桃青皮中芦丁、山奈酚的含量.docVIP

HPLC梯度洗脱法测定核桃青皮中芦丁、山奈酚的含量刘淑萍1，邸丁※ （1.河北联合大学化学工程学院，河北 唐山 063009；2.，μm）；流动相A相为乙腈，B相为pH=3的水溶液（用磷酸调节pH值），梯度洗脱；紫外检测器，检测波长为360nm；柱温25℃。用外标法定量，测定芦丁和山奈酚含量分别为0.5%、0.05%，分别在0.052~0.52μg(r=0.9982)和0.011~0.44μg(r=0.9991)范围内与色谱峰峰高呈良好的线性关系，平均回收率分别达98.84%，RSD=0.82%(n=6)和98.05%，RSD=2.06%(n=6)。该方法重复性好，各组分分离好。可以作为核桃青皮中的芦丁、山奈酚有效的分离和含量测定的方法。 关键词：核桃青皮；芦丁；山奈酚；HPLC；梯度洗脱；含量测定 中图分类号：S567.9 文献标识码：A Determination of rutin and kaempferol in walnut green husk by HPLC with gradient elution LIU Shu-ping1, DI Ding2※ College of Chemical Engineering, Hebei United University, Tangshan 063009, China; 2.Jiangsu Hansoh Pharmaceutical Group, Lianyungang HongChuang Pharmaceutical co., LTD, Lianyungang 222000, China） Abstract: To establish a HPLC method for simultaneous determinatioin of rutin and kaempferol in Walnut Green Husk. Rutin and kaempferol were identified from Walnut Green Husk, and rutin was identified for the first time. HPLC method was 收稿日期： 作者简介：刘淑萍（195—）E-mail：diding1513@163.comμm). The mobile phase was acetonitrile-pH=3 phosphoric acid, and with gradient elution. The detector was ultraviolet detector at 360nm. The column temperature was set 25℃. Contents of rutin and kaempferol were determined with extrnal standard method, and the contents were 0.5% and 0.05%. The calibration curves were linear within the range of 0.052~0.52μg (r=0.9982) and 0.011~0.44μg (r=0.9991) respectively and the average recovery rates of rutin and kaempferol were 98.84%, RSD=0.82% and 98.05%, RSD=2.06% (n=6). Repeatability of this method is good, and the various components are separated well. This method can be used as content determination method of rutin and kaempferol in Walnut Green Husk. Key words: walnut green husk; rutin; kaempferol; HPLC; gradient elution; content determinatioin 核桃又称胡桃（Julans Regia L），属胡桃科胡桃属植物。核桃青皮为核桃未成熟时外部的一层绿色果皮随意丢弃对环境很大核桃青皮核桃青皮核桃青皮1 试验部分 1.1 仪器与试剂UV765型紫外-可见分光光度计 均为分析纯用水为。 1.2 试验方法 1.2.1 对照品溶液的配制 μg/mL）分别为1.3，1.1；5.2，4.4；10.4，8.8；26.0，22.0；52.0，44.0的对照品混合溶液