银杏叶提取物EGB对CCl4肝损伤小鼠的MDA含量以及SOD活力的影响生物本科毕业论文.docVIP

银杏叶提取物EGB对CCl4肝损伤小鼠的MDA含量以及SOD活力的影响 摘要 目的：探讨银杏叶提取物EGB( 黄酮类) 对CCl4肝损伤小鼠以MDA含量及SOD活力的影响，生产出一种有效保护肝脏的药物。方法：清洁级昆明种小鼠♀♂兼用，随机分为空白对照组、吐温对照组、模型对照组、联苯双酯阳性对照组、实验组（银杏叶提取物低浓度、银杏叶提取物中浓度、银杏叶提取物高浓度），用四氯化碳皮下注射法建立慢性肝损伤模型，分别用生理盐水或药物灌胃治疗。检测肝组织中脂质过氧化物丙二醛（MDA）的含量和超氧化物歧化酶(SOD)的活力并加以探讨。结果：EGB能显著升高CCl4所致急性肝损伤小鼠其SOD活力，并可显著降低该模型小鼠肝组织中MDA的含量。结论：银杏叶提取物( 黄酮类)对此模型小鼠的肝损伤具有显著的保护作用，可能与降低、抑制脂质过氧化反应以及抗自由基损伤有关. 关键词 银杏叶提取物; CCl4肝损伤小鼠; MDA含量；SOD活力 Ginkgo biloba extract EGB CCl4 liver injury in mice on the MDA content and SOD activity of Abstract Objective: To investigate the Ginkgo biloba extract EGB (flavonoids) on CCl4 liver injury in mice with MDA content and SOD activity were to protect the liver to produce an effective drug. Methods: Kunming mice of clean grade ♀ ♂ altogether randomly divided into control group, Twain control group, model control group, DDB group, experimental group (low concentration of Ginkgo biloba extract, ginkgo biloba extract concentration, high concentrations of Ginkgo biloba extract), established by subcutaneous injection with carbon tetrachloride model of chronic liver injury, respectively, saline or drug the stomach. Detection of liver tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) of the activity and to be explored. Results: The significant increase can only CCl4 induced acute liver injury induced by the SOD activity, and significantly lower in the mice liver tissue MDA content. Conclusion: Ginkgo biloba extract (flavonoids) mouse model of liver injury have a significant protective effect may be related to decreased inhibition of lipid peroxidation and anti-free radical damage. Key words Ginkgo biloba extract; CCl4 liver injury in mice; MDA content; SOD activity  近几年来由于大量有害物质的产生，人们可能在不经意间，接触大量有害物质，例如CCL4, CCL4致急性中毒性肝炎、肝中毒四氯化碳进入肝细胞后，使线粒体膜的脂质溶解，从而影响线粒体的结构和功能，使酶蛋白质的合成减少，造成酶的破坏，因而影响代谢功能和能量的合成，致死。新观点认为细胞坏死的关键是由于四氯化碳抑制了细胞膜上钙泵活性，大量钙离子内流，堆积于细胞内的结果。此种动物模型可观察到急性肝炎的糖、脂肪、胞色素等方面的代谢障碍、肝解毒功能障碍，磺溴酚纳滞留血清，转氨酶明显升高，迅速出现营养不良、肝脂肪化变性及坏死等形态学变化。肝小叶中央坏死和肝细胞脂肪变性是其主要病变