斑马鱼管腔器官空腔形态发生及分子机制.pdfVIP

HEREDITAS (Beijing) 2013 4 , 35(4): 449―458 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2013.00449 斑马鱼管腔器官空腔形态发生及分子机制 肖春, 胡火珍, 莫显明 , , 610041 后生动物复杂的体内结构和器官结构多以网络状的管道系统出现。中空的管腔作为这个系统的重要结构 单元承担了运输物质、区分器官不同部位功能、分隔机体和外环境等诸多重要的生理功能。管腔的发育障碍将 致使相关器官形态发生畸形、功能紊乱。管腔型器官形态发生易被直接观察以及各种相关突变鱼和荧光转基因 鱼的出现, 使得斑马鱼(Danio rerio)成为管道器官研究的优秀模式动物。斑马鱼血管、神经管、小肠、胰腺外 分泌腺、前肾管等几种重要的器官的形态发生都伴随着典型的腔道发育过程, 是研究管腔形成的重要器官模 型。管腔形成由胞外信号诱导、细胞极性化、胞内物质定向运输、腔内液体形成和胞内细胞骨架重构等相关管 腔细胞内外发生的结构功能变化过程所构成, 而这些结构与功能的变化过程是通过精确而复杂的分子调控网 络来实现, 最终形成管道器官。文章对斑马鱼4 种典型管腔型器官的空腔形态发生过程进行了综述, 并总结了 此过程中的分子机制, 为今后的相关研究提供了参考。 管腔; 管腔器官; 极性; 细胞骨架 Lumen morphogenesis and molecular mechanisms in tubular organs during zebrafish embryonic development XIAO Chun, HU Huo-Zhen, MO Xian-Ming College of Life Sciences and Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China Abstract: A network tubular system is an important structure in the body and organ of metazoa. The lumen of tube is fundamental units in the structure, which serve to transport material, divide the organ into different functional compartments and separate the organ from the environment. The defects of lumen formation will lead to abnormalities of the organ morphogenesis and disorder of the function. Zebrafish (Danio rerio)is an important model for development research. Meanwhile easy observation of tubular organ, the relevant mutants, and transgene linages make zebrafish to become an excellent model to study the formation of lumen in the tub