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BiofoulingVol. 26, No. 3, April 2010, 301?312 The uronic acids assay: a method for the determination of chemical activity on bio?lm EPSKristina D.A. Mojica and Michael J. Cooney Department of Oceanography, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu, HI 96822, USA; bHawaii Natural Energy Institute, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu, HI 96822, USAReceived 7 June 2009; ?nal version received 20 November 2009 In this work, the uronic acids assay was evaluated for its potential to function as a bioassay to screen for antagonistic activity gainst the production of microbial bio?lm exopolysaccharideEPS. The assay was ?rst applied to bio?lms produced in the presence of two universal disinfectants sodium hypochlorite and sodium dodecyl sulfate known o inhibit microbial growth and bio?lm formation. The performance of the assay was the characterized through statistical assessment of threshold concentrations for disinfection e?ciency and consistency relative to values reported in the literature. The assay was then evaluated for its utility in screening for enzymatic or chemical inhibitors of bio?lm formation eg glycosidases, halogenated furanones, and semi-crude fractions extracted from minimally fouled marine plants and its ability to distinguish between true anti-bio?lm activity and simple disinfection. Activity was characterized as i no e?ect, ii a true positive e?ect ie increased bio?lm EPS, iii anti- bacterial activity ie decreased bio?lm EPS and analogous decrease in planktonic growth, and iv anti-bio?lm EPS activity ie decreased bio?lm EPS, without analogous decrease in planktonic growth. Results demonstrate that the uronic acids assay can augment existing bio?lm characterization methods by providing a quantitative measure of bio?lm EPS. Keywords: uronic acid; bio?lm; exopolysaccharide; assay; C. lytica Introduction 生物污染卷。 26日,第3期,2010年4月301-312糖醛酸的测定:用于化学活性生物膜每股收益克里斯蒂娜和Mich