疱疹病毒衣壳蛋白及其组装研究进展.pdfVIP

中国农业科学 2010,43(22): Scientia Agricultura Sinica 疱疹病毒衣壳蛋白及其组装研究进展 1 1，2 1，2 向 骏 , 程安春 , 汪铭书 1 2 ( 四川农业大学动物医学院，禽病防治研究中心，四川雅安 625014 ； 四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室，四川雅安 625014) 摘要：疱疹病毒衣壳主要由VP5蛋白构成，还包括其它3 种含量相对较少的蛋白，VP19C,VP23,VP26。VP5是 所有162个衣壳粒的结构亚单位，而VP19C和VP23则位于衣壳粒的空隙间。除了结构蛋白之外，衣壳的组装还涉 及蛋白酶和脚手架蛋白preVP22a的参与。冷冻电镜与重组杆状病毒技术的应用使得疱疹病毒衣壳的研究取得重要 进展，前者确认了衣壳的形态结构，后者通过证实完整衣壳组装过程中的中间体来确认了组装路径。本文综述了 当前疱疹病毒衣壳蛋白及其组装的研究进展。 关键词：疱疹病毒；衣壳；组装 Advance in Capsid Proteins and Assembly of Herpesvirus 1 1,2 1,2 XIANG Jun , CHENG An-chun , WANG Ming-shu 1 ( Research Center for Avian Disease, College of Veterinary Medicine ,Sichuan Agricultural University, Ya’an 625014, Sichuan ; 2College of Veterinary Medicine ,Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014, Sichuan) Abstract: The capsid of herpesvirus is composed of a major capsid protein called VP5 and three less abundant proteins, VP19C, VP23 and VP26. VP5 is the structural subunit of all 162 capsomers while VP19C and VP23 are located in the space between the capsomers. In addition to the structural proteins, capsid assembly involves participation of the protease and the scaffolding protein,preVP22a. Applying of cryoelectron microscopy and recombinant baculoviruses techniques have made considerable advances in understanding the capsid shell.The former has been allowed for a detailed analysis of the structure of the capsid. And the latter has been developed which has aided in defining the assembly pathway by identifying i