经股动脉冠状动脉球囊闭塞法建立犬心肌缺血再灌注模型.docVIP

  经股动脉冠状动脉球囊闭塞法建立犬心肌 缺血再灌注模型 1 2 1 2 2 1  5 10 15 20 25 30 35 40  （1. 中国医科大学附属第一医院老年病心内科 沈阳 110001； 2. 中国医科大学附属第一医院心内科 沈阳 110001） 摘要：【目的】探讨采用经股动脉冠状动脉球囊闭塞法，建立犬急性心肌缺血-再灌注模型 的可行性。【方法】杂种犬 24 只，麻醉后穿刺右股动脉，置入冠状动脉球囊至前降支的第 二对角支中、远端。充盈球囊堵塞冠状动脉。缺血 60min 后撤出球囊，再灌注 120min，建 立犬急性心肌缺血-再灌注模型。梗死区及正常区域取标本进行 TTC 大体染色及 HE 染色观察。 【结果】造模成功 19 只。1 只误堵塞左冠状动脉主干、2 只再灌注即刻发生室颤死亡，1 只 冠状动脉先天畸形，造影导管进入后左主干闭塞室颤死亡，1 只麻醉过量死亡。 【结论】经股动脉冠状动脉球囊闭塞法建立犬心肌缺血-再灌注模型可行，而且对实验动物 损伤小，对生理活动的影响与临床实际相似，更具有可比性。 关键词：心血管病学；心肌缺血再灌注；微创；动物模型 中图分类号：R-331 Minimally invasive close-chest method by the femoral artery for creating myocardial ischemia-reperfusion in dog REN Lina1, LI Yuze2, CHENG Ling3, SUN Yujiao2, LI Hua2, QI Guoxian1 (1. Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang, 110001; 2. Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, 110001) Abstract: Objectives: We sought to evaluate a closed-chest artery intubation through the femoral artery for reperfused myocardial infarction (MI) in dogs and to report experiences and pitfalls. Methods: In 24 dogs, a balloon catheter was advanced into the left descending coronary artery (LAD) under fluoroscopic guidance. In all other cases, the balloon was deflated after 60 minutes, and reperfused 120 minutes After the experiments were finished, the hearts were stained with 2,3,5-triphenyltetrazolium chloride and HE staining for assessment of infarct size. Results: Myocardial ischemia-reperfusion was successfully induced in 19 animals. One dog mistakenly left main coronary artery blockage and one dog anesthesia overdose deaths. Two dogs experienced ventricular fibrillation at the beginning of reperfusion and died, and one additional dog had a coronary artery malformation due to a particularly thin left main coronary artery and thus catheter blockage of the LAD caused ventricular fibrillation and immediate death. Conclusions: The described technique o