钙依赖性钾通道在鼻咽癌细胞调节性容积回缩中起重要作用.pdfVIP

生理学报 Acta Physiologica Sinica, October 25, 2009, 61 (5): 485-492 485 研究论文 钙依赖性钾通道在鼻咽癌细胞调节性容积回缩中起重要作用 1 2 1 1 3 1,* 2,* 和树同 ，朱林燕 ，杨林杰 ，何斯纯 ，毛建文 ，王立伟 ，陈丽新 1 2 3 暨南大学医学院 生理学系； 药理学系, 广州 510632 ； 广东药学院生物学教研室，广州 510006 摘 要：本研究采用膜片钳全细胞记录技术记录低渗诱发的人低分化鼻咽癌细胞(CNE-2Z)钾电流，用图像分析技术测量细 胞容积，探讨不同类型K+ 通道在调节性容积回缩(regulatory volume decrease, RVD) 中的作用。结果显示，细胞外灌流160 mOsm/L 低渗液诱发CNE-2Z 细胞产生容积激活性钾电流和RVD ，中等电导钙依赖性钾通道阻断剂clotrimazole (100 μmol/L)几 乎完全抑制低渗诱发的钾电流和RVD 。去除细胞外液的Ca2+ 后，低渗刺激不能诱发钾电流，RVD 显著减弱。电压依赖性 钾通道阻断剂4-aminopyridine (4-AP, 5 mmol/L)部分抑制低渗诱发的钾电流。上述结果提示，钙依赖性钾通道是低分化鼻咽 癌细胞容积激活性钾通道的主要类型；钙依赖性钾通道在鼻咽癌细胞RVD 中发挥了关键性作用，而电压依赖性钾通道在其中 起部分作用。 关键词：鼻咽肿瘤；低渗溶液；细胞大小；钾离子通道 中图分类号：R7 39. 63 Ca2+-dependent potassium channels play important roles in regulatory volume decrease in human nasopharyngeal carcinoma cells 1 2 1 1 3 1,* 2,* HE Shu-Tong , ZHU Lin-Yan , YANG Lin-Jie , HE Si-Chun , MAO Jian-Wen , WANG Li-Wei , CHEN Li-Xin 1Department of Physiology; 2 Department of Pharmacology, Medical College of Jinan University , Guangzhou 510632, China; 3Department of Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China Abstract: It has been shown that cell volume regulation mechanisms play important roles in various cell functions. We demonstrated previously that volume-activated chloride channels were involved in cell volume regulation. The present study aimed to clarify the roles of various types of potassium channels in regulatory volume decrease (RVD) induced by hypotonic challenges in human nasopharyn- geal carcinoma cells (CNE-2Z cells). The whole-cell patch clamp technique was used to record hypotonic challenge-induced pot