1类多巴胺受体活性变化对细胞凋亡FasFasL通路的影响.pdf

45 2 V o .l 45, N o. 2 20 11 4 JOURN AL O F HARB IN M ED ICA L U N IV ER S ITY A pr. , 20 11 103 1 Fas/ FasL 1 2 1 1 1 1 1 1 1 1* 李鸿珠 , 高 君 , 白淑芝, 李宏霞 , 王丽娜 , 李 弘, 张伟华 , 赵雅君 , 田 野 , 徐长庆 ( 1. 哈尔滨医科大学 病理生理学教研室, 黑龙江 哈尔滨 15008 1; 2. 哈尔滨市第一医院骨科, 黑龙江 哈尔滨 150010) [ ] 目的 ( F as/ F asL ) , 1( dopam ine recep or 1, DR 1) 方法 2 h 24 h 4 : , ( I /R ) , 10 m o l/ L SKF38393( DR 1)( SKF ) , 10 mo l/ L SCH 23390 ( DR 1)( SCH ) RTPCR W es ern blo FasF asLCaspase8C aspase3 m R NA ; TUN EL ; ( lac a e dehydrogenase, LDH ) ( superox ide dismu ase, SOD ) ( m a lond ialdehyde, M DA ) ; 结果 , I/ R F as FasLcaspase8caspase3, LDH M DA , SOD , ; I /R , 10 mo l/ L SK F38393, 10 m o l/L SCH23390 结论 DR 1, Fas /F asL [ ] 1; ; ; F as/ FasL [ ] R 587 [ ] A [] 1000 - 1905( 20 11) 02- 0 103- 06 Effect of dopamine receptor1 activation on Fas/FasL pathway of apoptosis 1 2 1 1 1 1 L I H ongzhu , GAO Jun , BA I Shuzh i, L IH ongx ia , WANG L ina , L I H ong , ZHANG W e i 1 1 1 1 hua , ZHAO Y ajun , T IAN Y e , XU Changqing ( 1. D epartment of Pathophysiology, H arbin M edical University, H arbin 150081, China; 2. D epart m ent of Orthopaedics, The irstH osp ital of H arbin,H arbin 150010, China) Abstract: Objective T o inves iga e he effec and possib le m echanism s of dopam ine recep or1 ( DR 1) ac iva ion on card iomyocy es apop osis induced by ischem iareperfu sion of prmi ary neona al ra ven ricular cardiom yocy es. Methods The ischem iareperfu sion ( I/R) in jury m ode lw as es ab lished by incuba ion in ischem iam mi e ic so lu ion for 2 h, and reincuba ion in norm a l cul ure m ed i um for 24 h. C ells w ere