补肾活血饮对帕金森病大鼠脑内多巴胺D2受体含量的影响.pdfVIP

2011;31(11) 南方医科大学学报（J South Med Univ ） ·1879 · ·基础研究· 补肾活血饮对帕金森病大鼠脑内多巴胺D2 受体含量的影响 王海明，杨明会，窦永起，刘 毅，李绍旦，李 敏 解放军总医院中医研究所，北京 100853 摘要：目的探讨补肾活血饮治疗帕金森病(PD)的作用机制。方法 用直接向脑组织内注入6-羟基多巴损毁脑黑质致密部 的方法建立PD 大鼠模型。将120只SD大鼠随机分为正常对照组、生理盐水模型组和补肾活血饮治疗组，观察各组PD 大 鼠异常不自主运动变化；用放射免疫法测定治疗后大鼠脑组织多巴胺D2 受体（DRD2）平衡解离常数（KD）和最大结合量 （Bmax ）的变化；免疫组化法观察脑组织DRD2 受体阳性细胞数。结果 补肾活血饮组大鼠的异常不自主运动较模型组减 少。补肾活血饮治疗组大鼠损毁侧脑组织DRD2 Bmax 较模型组显著增高（P0.01），KD 值较模型组下降（P0.01）；DRD2 受体阳性细胞数（80.9± 13.59）较模型组（11.15±6.78）明显升高（P0.01），但与对照组无显著差异（P0.05）。结论 补肾活 血饮能改善PD 大鼠不自主运动，促进PD 模型大鼠脑组织DRD2 表达，提高DRD2 亲和力。 关键词：帕金森病；补肾活血饮；多巴胺D2 受体 中图分类号：R289.3; R742 文献标志码：A 文章编号：1673-4254（2011）11-1879-03 Effect of Bushen Huoxue Decoction on dopamine D2 receptor in the brain of rats with Parkinsons disease WANG Hai-ming, YANG Ming-hui, DOU Yong-qi, LIU Yi, LI Shao-dan, LI Min Department of Traditional Chinese Medicine, General Hospital of PLA, Beijing 100853, China Abstract: Objective To explore the mechanism underlying the therapeutic effect of Bushen Huoxue Decoction (BHD), a traditional Chinese medicinal preparation, on dopamine D2 receptor (DRD2) in the brain of rat models of Parkinsons disease (PD). Methods A total of 120 SD rats were randomized into normal control group, saline model group and BHD-treated group. In the latter two groups, PD rat models were established by direct injection of 6-OHDA to destruct the substantia nigra compact part (SNC) with corresponding treatments. The behavioral changes of the rats were observed. Radioimmunoassay was employed to determine the changes in the equilibrium dissociation constant (Kd) and maximal binding capacity (Bmax) of DRD2, and immunohistochemistry