因子、γ-干扰素影响.pdfVIP

·90 · 南方医科大学学报（JSouthMedUniv） 2011;31(1) ·基础研究· 补肾活血饮对帕金森病模型小鼠脑组织一氧化氮及α-肿瘤坏死 因子、γ-干扰素的影响 李绍旦，刘 毅，杨明会 解放军总医院中医研究所，北京 100853 摘要：目的探讨补肾活血饮对帕金森病模型小鼠脑组织中NO 及细胞因子α-肿瘤坏死因子（TNF-α）、γ-干扰素（IFN-γ）的 影响。方法 45 只C57BL/6 小鼠随机分为生理盐水正常组、1-甲基-4-苯基-1,2,3,6 四氢吡啶（MPTP）模型组和补肾活血饮 中药组，用分光光度法和ELISA 法分别检测各组小鼠脑组织NO 及细胞因子TNF-α、IFN-γ含量。结果模型组NO 含量为 （5.93±0.79）μmol/g protein ，高于正常组（P0.01）和中药组（P0.01）。模型组TNF-α、IFN-γ含量分别为（0.36±0.11）ng/L 和 （0.83±0.25）ng/L ，高于正常组（P0.001）和中药组（P0.01）。中药组和正常组NO 及TNF-α、IFN-γ含量比较均无显著性差 异（P0.05）。结论 补肾活血饮治疗帕金森病的疗效机制可能与其能降低NO 及TNF-α、IFN-γ含量有关。 关键词：帕金森病；补肾活血饮；一氧化氮；肿瘤坏死因子-α；干扰素-γ 中图分类号：R285 文献标志码：A 文章编号：1673-4254（2011）01-0090-03 Effect of Bushenhuoxue Yin on cerebral levels of nitric oxide, tumor necrosis factor-αand interferon-γin a mouse model of Parkinson disease LI Shao-dan, LIU Yi, YANG Ming-hui Institute of Traditional Chinese Medicine, General Hospital of PLA, Beijing 100853, China Abstract: Objective To observe the effects of Bushenhuoxue Yin (BSHXY), a compound traditional Chinese herbal medicine, on nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) levels in the brain of a mouse model of Parkinson disease (PD). Methods Forty-five C57BL/6 mice were randomized into MPTP-induced PD model group, BSHXY treatment group and normal control group. The contents of NO and the two cytokines in the brain tissue of the mice were determined by spectroscopy and enzyme-linked immunosorbent assay, respectively. Results The concentration of NO in the brain tissue of the PD model group was 5.93±0.79 μmol/tein, significantly higher than that in BSHXY group